骨髓间充质干细胞移植对大鼠重症急性胰腺炎的治疗作用观察

摘要

目的：探讨骨髓间充质干细胞（ MSCs）移植对重症胰腺炎（ SAP）的治疗作用及相关机制。方法将雄性SD大鼠120只随机分为假手术组、模型组及移植组各40只。模型组和移植组采用5％牛磺胆酸钠逆行胰胆管注射方法制作SAP模型，假手术组开腹后翻动肠壁即关腹。造模成功后移植组经门静脉植入MSCs；模型组及假手术组采用同样方法给予等量生理盐水。于24、48、72 h每组分别处死10只大鼠，对胰腺和肾脏损伤进行大体形态和光镜下观察，测定血清淀粉酶（ AMY）、BUN、Cr、TNF-α和IL-6水平。结果模型组胰腺和肾脏组织损伤明显，各时间点血清AMY、BUN、Cr、TNF-α和IL-6水平均高于假手术组，P均＜0．05。移植组胰腺和肾脏组织损伤有所减轻；各时间点血清中AMY、BUN、Cr、TNF-α和IL-6水平均低于模型组及假手术组，P均＜0．05。结论经门静脉移植MSCs治疗SAP效果确切，可降低多器官功能障碍的发生率。%Objective To study the protective effect and the possible mechanisms of transplantation bone marrow mes-enchymal stem cells (MSCs) on pancreatic injury in rats with severe acute pancreatitis(SAP).Methods A total of 120 male Sprague-Dawley rats were randomly divided into sham operation group( N group) , severe acute pancreatitis group ( P group) , transplantation MSCs group( M group) .The dose of 5%sodium taurocholate was retrograde injected into the pan-creatic duct of the rats in P group and M group to induce SAP model.Rats in N group whose pancreases were just flipped and striked gently without injection.MSCs were transplanted into rats in M group via portal vein, while the same dosage of 0.9%NS was injected by the same vein routine in P group.Ten rats in each group were sacrificed at 24 h, 48 h, 72 h lat-er.Pancreas and kidney damage of each group were observed under gross morphology and optical lens.The serum AMY, BUN, Cr, TNF-αand IL-6 level of each group were tested.Results Pancreas and kidney injury in P group were signifi-cantly heavier.The content of serum AMY, BUN, Cr, TNF-αand IL-6 level in P group were both higher than those of N group(P<0.05).Pancreatic and renal tissue injury in M group rats mitigated.The content of serum AMY, BUN, Cr, TNF-αand IL-6 level in M group were both lower than those of P group (P<0.05).Conclusions Transplantation via portal vein MSCs is an effective treatment on SAP, which can reduce the occurrence of multiple organ dysfunction and im-prove prognosis.