Objective To investigate the impaction of sufentanil post-conditioning on Cx43 expression of myocardial ischemia reperfusion injury ( I/RI) .Methods Sixty Sprague Dawley( SD) rats were randomly divided into 4 groups ( n=15).The group B:Ligature the left anterior descending coronary artery (LAD) of rat heart for 30 min of ischemia, then 120 min of reperfusion, the myocardial ischemia-reperfusion injury model could be obtained; the group A: only thread, without ligation of LAD for 150 min;the group C:ischemia 30 min line at the end of the 30 s, reperfusion 30 s, repeat 3 times, 120 min of reperfusion;the group D:5 min before 30 min of ischemia reperfusion intravenous drip sufentanil 0.1μg/kg, 120 min of reperfusion.Took out rat heart at the end of reperfusion, then calculated the percentage of infarct size of myocardium ( MIS%) by triphenyltet tetrazolium chloride ( TTC) staining, and observed the content of total connexin 43 (tCx43) and phosphorylated connexin 43 (pCx43) in myocardium by semiquantitative Western blotting.Results The MIS%of group A, B, C, D were respectively 0.8%±2.4%, 44.6%±5.3%, 37.4%±3.7%, 26.5%±4.2%, com-pared with group B and group C, MIS% in the group D was lower significantly, P<0.05.Basing on the electrophoresis banding density of tCx43 and pCx43 of group B, the percentage of density of tCx43 of group A, B, C, D were respectively 2.3, 1.0, 1.3, 1.8;the percentage of density of pCx43 of group A, B, C, D were respectively 2.4, 1.0, 1.3, 1.6. There was little expression of tCx43 and pCx43 in group B;the expression of tCx43 and pCx43 in group D was higher signif-icatively than that in group B and group C (P<0.05).Conclusion Sufentanil may play a protective role on rat myocardi-al ischemia reperfusion injury by increasing the content of Cx43 of rat myocardium after the myocardial ischemia reperfu-sion.%目的：观察舒芬太尼后处理对缺血/再灌注大鼠心肌线粒体Cx43蛋白表达的影响。方法将60只SD大鼠随机分为A、B、C、D组各15只，B组结扎大鼠左冠状动脉前降支（ LAD）30 min，松开结扎线灌注120 min，制作心肌缺血/再灌注模型；A组只穿线，不结扎LAD，持续150 min；C组缺血30 min末行缺血30 s，再灌注30 s，重复3次，再灌注120 min；D组缺血30 min、再灌注前5 min静滴舒芬太尼0．1μg/kg，再灌注120 min。再灌注末取大鼠心脏，TTC法染色心肌切片并计算心肌梗死面积百分比（ MIS％）；Western blotting半定量法测定心肌线粒体总Cx43（tCx43）和磷酸化Cx43（pCx43）表达。结果 A、B、C、D组的MIS％分别为0．8％±2．4％、44．6％±5．3％、37．4％±3．7％、26．5％±4．2％，D组与B、C组相比，P均＜0．05。以B组心肌线粒体tCx43和pCx43电泳条带灰度为基准，A、B、C、D组心肌线粒体tCx43灰度比分别为2．3、1．0、1．3、1．8；pCx43灰度比分别为2．4、1．0、1．3、1．6。 B组有少量tCx43和pCx43蛋白表达；D组tCx43和pCx43蛋白表达明显高于B、C组（ P均＜0．05）。结论舒芬太尼可能通过增加缺血/再灌注大鼠心肌线粒体Cx43蛋白表达发挥心肌保护作用。
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