miR-146a基因多态性与腔隙性脑梗死的关系

摘要

目的：探讨miR-146a基因多态性与腔隙性脑梗死（ LI）的关系。方法选择LI患者173例（观察组）和与其性别、年龄匹配的非心脑血管疾病体检者298例（对照组），采用SNaPshot 法检测血miR-146a rs2910164和rs57095329位点的基因多态性，B型彩色多普勒超声仪检测颈动脉内膜中层厚度（CIMT）。结果观察组miR-146a rs2910164位点GG基因型频率为8．67％、CC＋CG基因型频率为91．33％、G等位基因频率为33．24％，对照组分别为17．11％、82．89％、39．93％，P均＜0．05。两组miR-146a rs57095329基因型和等位基因频率比较，P均＞0．05。观察组70岁以下者miR-146a rs2910164位点G等位基因频率为28．57％，对照组为39．12％，P＜0．05。观察组miR-146a rs2910164和rs57095329位点正常和突变基因型患者的CIMT比较，P均＞0．05。结论 LI患者miR-146a基因rs2910164位点G等位基因频率降低，G等位基因可能是其发病的一种保护性因素。%Objective To investigate the association between miR-146a gene polymorphism and lacunar infarction ( LI) .Methods The present study recruited 173 patients with LI ( observation group ) and 298 age-and sex-matched out-patient controls without cardiovascular and cerebrovascular diseases ( control group ) .Multiplex SNaPshot was employed to determine the genotype and allele frequencies of rs 2910164 and rs57095329 polymorphisms of the miR-146a gene.B-color Doppler ultrasonography was used to determine the carotid artery intima-media thickness ( CIMT) .Results The frequen-cies of the GG genotype , CC+CG genotype and G allele in the observation group were 8.67%, 91.33%and 33.24%, re-spectively;While the frequencies in the control group were 17.11%, 82.89%and 39.93%, respectively (all P<0.05). The genotype and allele frequencies of rs57095329 were not statistically associated with LI (all P>0.05).The frequency in patients below 70 years old of the observation group carrying the G allele of miR-146a rs2910164 polymorphism was 28.57%, and the frequency in the control group was 39.12% (P<0.05).The CIMT of common genotype and mutant genotype between rs2910164 and rs57095329 polymorphisms of miR-146a in the observation group was not significantly dif-ferent (all P>0.05).Conclusion The present study suggests that the frequency of the G allele of miR-146a rs2910164 is lower in LI patients and the rs 2910164 G allele is likely to play a protective role in the etiology of lacunar infarction .