非小细胞肺癌组织中磷酸甘油酸变位酶1表达及其与患者预后的关系

摘要

目的：探讨非小细胞肺癌（NSCLC）组织中磷酸甘油酸变位酶1（PGAM1）的表达及其与患者预后的关系。方法选择NSCLC患者206例，采用Real－time PCR技术检测其肿瘤组织中PGAM1 mRNA表达，其中28例患者配对检测其癌旁正常组织中PGAM1 mRNA表达。以ROC曲线中约登指数最高值（1．96）作为PGAM1 mRNA表达水平的分割点，＞1．96为PGAM1 mRNA高表达，≤1．96为PGAM1 mRNA低表达。分析NSCLC组织中不同PGAM1 mRNA表达与患者临床病理特征的关系；采用Kaplan－Meier生存分析法计算不同PGAM1 mRNA表达者的总生存期（ OS）、无病生存期（ DFS），并采用Cox比例风险回归模型分析患者OS、DFS的影响因素。结果28例NSCLC患者癌组织中PGAM1 mRNA表达明显高于其癌旁正常组织（P＜0．05）。 PGAM1 mRNA表达与NSCLC患者吸烟状况、组织学类型、病理学T分级有关（P均＜0．05）。 Kaplan－Meier生存分析显示，PGAM1 mRNA高表达者OS、DFS中位时间均低于PGAM1 mRNA低表达者（P均＜0．01）。 Cox比例风险回归模型分析显示，PGAM1 mRNA高表达是OS、DFS的独立影响因素（HR分别为1．730、1．698，P均＜0．05）。结论 PGAM1 mRNA在NSCLC组织中表达升高，其高表达提示患者预后不良。%Objective To investigate the expression of phosphoglycerate mutase 1 ( PGAM1 ) in patients with non-small-cell lung cancer ( NSCLC) and its correlation with the prognosis.Methods We measured PGAM1 mRNA expression level in the tumor tissues of 206 cases of NSCLC patients by real-time PCR, of which 28 patients were detected the PGAM1 mRNA expression in the matched adjacent normal tissues.We defined the expression of PGAM1 mRNA>1.96 as the high expression group, PGAM1 mRNA≤1.96 as the low expression group, the correlations of PGAM1 mRNA expression in NSCLC tumor tissues with clinicopathologic characeristics were analyzed.Kaplan-Meier survival analysis was used to evaluate the overall survival ( OS) and disease-free survival ( DFS) of PGAM1 mRNA in the high expression group and low expression group.And the Cox regression models were used to analyze the risk factors influencing the patients′OS and DFS.Results The PGAM1 mRNA expression was significantly higher in 28 cases of NSCLC tumor tissues than their matched normal tissues (P<0.05).The PGAM1 mRNA expression was related with smoking, histological type and advanced T stage (all P<0.05).Kaplan-Meier analysis revealed the median time of OS and DFS in PGAM1 mRNA high expression group was lower than that of PGAM1 mRNA low expression group (all P<0.01).Cox regression analysis indicated that PGAM1 mRNA was an independent risk factor for OS (HR=1.730, P<0.05) and DFS (HR=1.698, P<0.05).Conclusion The expression of PGAM1 mRNA in NSCLC tissues was increased, and its high expression indicates their poor prognosis.