大蒜素防治大鼠肝纤维化的效果及其机制探讨

摘要

Objective To observe the protective effect of allicin on hepatic fibrosis in rats , and to investigate its mechanism.Methods Forty-five Wistar rats were randomly divided into six groups as follows: blank control group , sol-vent control group , model group , low-dose allicin prevention group , medium-dose allicin prevention group and medium-dose allicin treatment group .Rat models of hepatic fibrosis in the model , low-dose prevention allicin , medium-dose preven-tion allicin and medium-dose allicin treatment groups were induced by carbon tetrachloride ( CCl4 ) intraperitoneally for eight weeks.Rats in the low-dose and medium-dose allicin prevention groups received allicin (11, 22 mg/(kg· d)) at the beginning of modeling.Rats in the medium-dose allicin treatment group received allicin (22 mg/(kg· d)) from the fifth week.Rats in the solvent control group was treated with the same amount of vegetable oil for 8 weeks.The expression levels of ALT, AST and transforming growth factor-β( TGF-β) were detected by ELISA .The hepatic histological changes were also observed by optical microscopy as well as the hyperplasia degree of collagen fiber .Results Comparison of ALT , AST serum levels:model group >low-dose allicin prevention group >medium-dose allicin prevention group , medium-dose al-licin treatment group, blank control group, or solvent control group (all P<0.05).Comparison of TGF-βserum levels:model group >low-dose allicin prevention group >medium-dose allicin prevention group >medium-dose allicin treat-ment group >blank control group or solvent control group (all P<0.05).The histopathological results showed that allicin could attenuate the degree of hepatic fibrosis and cellular degeneration and protect the entire structure of liver tissues .Com-parison of liver cell degeneration degree and collagen fiber hyperplasia degree evaluation :model group >low-dose allicin prevention group , medium-dose allicin prevention group , medium-dose allicin treatment group >blank control group , sol-vent control group ( all P<0.01).Conclusion The medium-dose allicin can prevent the hepatic fibrosis of rats with mechanism of down-regulating the TGF-βexpression and reducing the degeneration of liver cells .%目的：观察大蒜素对大鼠肝纤维化的防治效果，并探讨其机制。方法将45只大鼠随机分为6组，其中模型组、小剂量预防组、中剂量预防组及中剂量治疗组均采用四氯化碳（ CCl 4）腹腔注射的方法建立大鼠肝纤维化模型，小、中剂量预防组于造模开始分别给予大蒜素11、22 mg／（ kg· d）灌胃，中剂量治疗组则于造模后第5周给予大蒜素22 mg／（ kg· d）灌胃；空白对照组不做处理，溶剂对照组给予等量植物油灌胃及腹腔注射，共8周。采用ELISA法检测各组血清ALT、AST、生长转化因子β（ TGF-β），光镜下观察肝细胞变性、胶原纤维增生程度并进行评分。结果各组血清ALT、AST比较，模型组＞小剂量预防组＞中剂量预防组、中剂量治疗组、空白对照组、溶剂对照组，P均＜0．05。各组血清TGF-β比较，模型组＞小剂量预防组＞中剂量预防组＞中剂量治疗组＞空白对照组、溶剂对照组，P均＜0．05。病理组织学检查结果显示，小、中剂量预防组及中剂量治疗组肝细胞变性和纤维化程度明显减轻，肝组织结构比较完好。各组肝细胞变性程度及胶原纤维增生程度评分比较，模型组＞小剂量预防组、中剂量预防组、中剂量治疗组＞空白对照组、溶剂对照组，P均＜0．01。结论中等剂量的大蒜素可较好地防治大鼠肝纤维化，可能与其降低TGF-β表达及减少肝细胞变性有关。