创伤后应激障碍患者外周血单个核细胞的p11 mRNA 表达变化及意义

摘要

目的：观察创伤后应激障碍（ PTSD）患者外周血单个核细胞p11 mRNA表达变化，并探讨其意义。方法PTSD患者19例（ PTSD组），经历创伤事件但未发生PTSD的患者13例（非PTSD组），健康体检者19例（对照组）。采用实时荧光定量聚合酶链式反应检测各组p11 mRNA，以创伤后应激障碍量表平民版（ PCL-C）、汉密顿焦虑量表（HAMA）、汉密顿抑郁量表（HAMD）评估患者病情，Pearson 相关分析法分析p11 mRNA表达与HAMA、HAMD、PCL-C评分的相关性。结果 PTSD组、非PTSD组、对照组p11 mRNA相对表达量分别为－0．495±0．920、0．911±1．474、－0．000±0．791，PTSD组、对照组分别与非PTSD组比较，P均＜0．05。 PTSD 组HAMA、HAMD、PCL-C评分分别为（21．63±7．79）、（25．11±8．56）、（55．84±6．35）分，非PTSD组分别为（10．31±6．22）、（13．00±9．88）、（31．77±5．09）分，两两比较，P均＜0．05。 p11 mRNA相对表达量与HAMD评分呈负相关（r＝－0．299，P＝0．033），与PCL-C评分呈负相关（r＝－0．385，P＝0．030），与HAMA评分无相关性（r＝－0．233，P＝0．111）。结论 PTSD患者p11 mRNA表达降低，检测此指标有助于鉴别诊断PTSD与非PTSD。%Objective To investigate the expression changes and significance of p 11 mRNA in the peripheral blood mononuclear cells (PBMCs) of patients with post-traumatic stress disorder (PTSD).Methods In the PTSD group, there were 19 cases of patients;during the 19 patients, 13 cases of patients (who were taken as the non-PTSD group) did not de-veloped into PTSD;another 19 cases of healthy people were selected as the control group .The mRNA expression levels of p11 of all participants were estimated by using real-time fluorescence quantitative polymerase chain reaction .Post-traumatic stress disorder check-list-civilian version ( PCL-C), Hamilton Anxiety Scale ( HAMA) and Hamilton Depression Scale ( HAMD) were used to access the condition of patients .Pearson correlation analysis was used to analyze the correlations of p11 mRNA expression with HAMA, HAMD and PCL-C scores.Results The relative expression of p11 mRNA in the PTSD group, non-PTSD group and control group was -0.495 ±0.920, 0.911 ±1.474 and -0.000 ±0.791, respective-ly.The PTSD group and control group showed a significant difference as compared with the non -PTSD group ( all P<0.05).The scores of HAMA, HAMD and PCL-C in the PTSD group were (21.63 ±7.79), (25.11 ±8.56) and (55.84 ±6.35), respectively.In the non-PTSD group, the scores were (10.31 ±6.22), (13.00 ±9.88) and (31.77 ±5.09), respectively.Multiple comparison showed there were significant differences between these two groups (all P<0.05).In the PBMCs, the relative expression of p11 mRNA was negatively correlated with HAMD (r=-0.299, P=0.033), was negatively correlated with PCL-C (r=-0.385, P=0.030), and was not related with HAMA (r=-0.233, P=0.111). Conclusion The p11 mRNA expression level is decreased , which may help in the diagnosis and differential diagnosis of PTSD and non-PTSD.