新疆维吾尔族人群中MTHFR、MTRR基因多态性与NAFLD的相关性

摘要

目的：探讨新疆维吾尔族人群5，10-亚甲基四氢叶酸还原酶（MTHFR）及甲硫氨酸合成酶还原酶（MTRR）基因多态性与非酒精性脂肪肝（NAFLD）的相关性。方法选取维吾尔族 NAFLD 患者325例作为NAFLD组，选择健康体检的维吾尔族居民304例作为非NAFLD组，对研究对象进行问卷调查、体格检查以及血液生化指标的检测。应用多重高温连接酶检测反应技术检测两组MTHFR 基因C677T、A1298C位点及MTRR基因A66G位点的多态性。采用Logistic 多因素回归法分析维吾尔族 NAFLD与 MTHFR 基因 C677T、A1298C 位点及MTRR基因A66G位点多态性的相关性。结果两组MTHFR 基因C677T、A1298C位点和MTRR基因A66G位点基因型及等位基因的分布差异均无统计学意义（P均＞0．05）。调整混杂因素后，Logistic回归分析显示，MTHFR基因A1298C基因位点在隐性模型下与NAFLD有相关趋势（OR＝2．023、P＜0．05），在其余加性模型和显性模型的基因型分布差异无统计学意义（P均＞0．05）。MTHFR 基因C677T位点、MTRR基因A66G位点的加性模型、显性模型和隐性模型下的基因型分布差异均无统计学意义（P均＞0．05）。结论新疆维吾尔族人群中MTHFR基因A1298C基因位点多态性与NAFLD的发生有关，AA基因型可能增加NAFLD的发病风险。%Objective To investigate the correlations between the methylenetetrahydrofolate reductase (MTHFR)and methionine synthase reductase (MTRR)gene polymorphisms and non-alcoholic fatty liver disease (NAFLD)in Uygur pop-ulations from Xinjiang.Methods Totally 325 Uygur patients with NAFLD (NAFLD group)and 304 healthy controls from physical checkups were selected.Questionnaire survey,physical examination and detections of blood biochemical in-dexes were conducted.The gene polymorphisms of MTHFR C677T and A1298C and MTRR A66G in the two groups were detected by improved multiple ligase detection reaction.The correlations between NAFLD and gene polymorphisms of MTH-FR C677T and A1298C and MTRR A66G in Uygur populations from Xinjiang were analyzed by multivariate Logistic regres-sion.Results No significant difference was found in the genotype and allelic distribution of MTHFR C677T and A1298C and MTRR A66G between the NAFLD and the control group (P>0.05).After adjusting confounding factors,an associa-tion of MTHFR A1298C with NAFLD was observed in the recessive model (OR=2.023,P<0.05),while in the additive and dominant models,no statistically significant difference was found (P>0.05 ).No statistically significant difference was found in the genotype distribution of MTHFR 677T and MTRR A66G in the additive model,dominant model and reces-sive model (P>0.05 ).Conclusion The gene polymorphism of MTHFR A1298C is associated with the occurrence of NAFLD in Xinjiang Uygur population and AA genotype may increase the risk of NAFLD.