五味子多糖对人脑胶质瘤 U87细胞活力的影响及其机制

摘要

Objective To observe the effects of schisandra chinensis polysaccharides ( CPSC) on the cell activity of human brain glioma U87 cells, and to explore its possible mechanism.Methods The human glioma U87 cells in the loga-rithmic growth phase were divided into groups A, B, C and control group.The cells in the groups A, B and C were added with the final concentrations of 200, 400 and 800μg/mL of CPSP to culture.The control group was treated with the equal volume of cell culture fluid.The cell viability was observed at 24, 48 and 72 h after culture.At 48 h, the cysteine aspartic acid proteinase 3 (Caspase-3) in the nutrient solution supernatant was detected.Results At 24, 48 and 72 h, the cell survival rates of group A were respectively 1.17 ±0.04, 1.09 ±0.05 and 1.39 ±0.05.The cell survival rates of group B were respectively 1.01 ±0.03, 1.01 ±0.03 and 1.21 ±0.02.The cell survival rates of group C were respectively 0.90 ± 0.02, 0.76 ±0.02 and 0.98 ±0.03.The cell survival rates of the control group were respectively 1.28 ±0.03, 1.16 ± 0.03 and 1.45 ±0.01.At 72 h, the cell survival rate of group B was statistically significant as compared with that of the control group (P<0.05).Significant difference was found in the survival rate between group C and control group at 24, 48 and 72 h (P<0.05).The relative expression of Caspase-3 protein in cultural supernatant of groups A, B, C and control group was respectively 0.79 ±0.02, 0.96 ±0.01, 1.13 ±0.01 and 0.78 ±0.01, and significant difference was found be-tween the groups B, C and the control group (all P<0.05).Conclusion High concentration of CPSC can inhibit the vi-ability of U87 cells, and its mechanism may be that cpsc promote the Caspase-3 expression.%目的：观察五味子多糖（ CPSC）对人脑胶质瘤U87细胞活力的影响，并探讨其可能作用机制。方法取适量对数生长期人脑胶质瘤U87细胞，分为A、B、C组和对照组，A、B、C组分别加入终浓度为200、400、800μg／mL的CPSP培养，对照组加入等体积细胞培养液培养，分别于培养24、48、72 h时观察各组细胞活力；培养48 h时取各组培养液上清，检测半胱氨酸天冬氨酸蛋白酶3（Caspase-3）。结果培养24、48、72 h时，A组细胞存活率分别为1．17±0．04、1．09±0．05、1．39±0．05，B组分别为1．01±0．03、1．01±0．03、1．21±0．02，C组分别为0．90±0．02、0．76±0．02、0．98±0．03，对照组分别为1．28±0．03、1．16±0．03、1．45±0．01；培养72 h时，B组细胞存活率与对照组相比，P＜0．05；培养24、48、72 h时，C组细胞存活率与对照组相比，P均＜0．05。培养48 h时A、B、C组及对照组培养液上清Caspase-3蛋白的相对表达量分别为0．79±0．02、0．96±0．01、1．13±0．01、0．78±0．01，B、C组与对照组相比，P均＜0．05。结论高浓度CPSC可抑制U87细胞的活力，其机制可能是与CPSC促进细胞Caspase-3表达有关。