Objective To observe the expression changes of phosphorylated mammalian target of rapamycin (p-mTOR) and p70s6k protein in non-small-cell lung cancer (NSCLC) tissues and to discuss its clinical significance .Meth-ods Eighty-two cases of patients with NSCLC were selected .The tumor tissues obtained from surgery (lung cancer group) , 46 cases of tissue samples from alveolus healthy tissues (control group 1) , and 50 cases of tissue samples from bronchus mucosal tissues (control group 2) were collected.The expression of p-mTOR and p70s6k protein in the three groups was measured by immunohistochemistry and we analyzed the relationships with the clinical and pathological data . Results The positive expression rates of p-mTOR protein in the lung cancer group , control group 1 and control group 2 were 68.29%, 17.39% and 32.0%, respectively.The positive expression rates of p70s6k protein in the lung cancer group, control group 1 and control group 2 were 85.37%, 21.74%and 32.00%, respectively.Significant difference was found between the lung cancer group and the control group 1, control group 2 (all P＜0.01).The expression of p-mTOR protein in poorly differentiated cancer tissues was significantly higher than that of medium and high differentiation cancer tis -sues (P＜0.01).In the NSCLC tissues, the p-mTOR and p70s6k expression was positively correlated (r=0.210, P=0.0032).Conclusion p-mTOR and p70s6k protein is highly expressed in NSCLC tissues , which may be used as the early-warning index in the early diagnosis or as a new target for anti-cancer drug.%目的 观察非小细胞肺癌组织中磷酸化雷帕霉素靶蛋白(p-mTOR)及核糖体40s小亚基S6蛋白激酶(p70s6k)蛋白的表达变化,并探讨其意义.方法 非小细胞肺癌患者82例,手术切除肿瘤组织作为肺癌组,取患者肺癌同侧远端的肺泡正常组织46例份作为对照组1,正常支气管黏膜组织50例份为对照组2.免疫组化方法 检测3组组织中的p-mTOR及p70s6k蛋白,并分析其与非小细胞肺癌临床病理参数的关系.结果 肺癌组、对照组1、对照组2 p-mTOR蛋白表达阳性率分别为68.29%、17.39%、32.0%,p70s6k蛋白表达阳性率分别为85.37%、21.74%、32.00%,肺癌组与对照组1、对照组2相比,P均＜0.01.非小细胞肺癌低分化p-mTOR蛋白表达阳性率高于中高分化(P＜0.01).肺癌组织中p-mTOR和p70s6k蛋白的表达呈正相关(r=0.21,P=0.0032).结论 p-mTOR、p70s6k蛋白在非小细胞肺癌组织中高表达,可作为早期诊断肺癌的指标或抗癌药物作用的新靶点.
展开▼
