Objective To explore the expression changes of the transcription factor Twist and the E-cadherin in the o-ral squamous cell carcinoma (OSCC)tissues and to investigate the clinical significance. Methods The immunohisto-chemical SP method was used to detect the expression levels of the Twist and E-cadherin in 65 cases of OSCC tissues and 45 cases of oral normal mucosa tissues. In addition,the relationship between the expression of Twist/ E-cadherin and the clinicpathologic characteristics was analyzed,and the Spearman was used to explore the correlation between the Twist and E-cadherin in OSCC tissues. Results The positive expression rates of Twist in the OSCC tissues and oral normal mucosa tissues were 72. 31% (47 / 65)and 11. 11% (5 / 45),respectively (P < 0. 05). The positive expression rates of E-cadher-in in the OSCC tissues and oral normal mucosa tissues were 23. 08% (19 / 65)and 86. 67% (39 / 45),respectively (P <0. 05). The Twist and E-cadherin expression levels in the OSCC tissues were related with the lymph node metastasis and the clinical stages (both P < 0. 05),but were not related with the gender,age,differentiation degree and the type (all P >0. 05). The expression of Twist and E-cadherin was negatively correlated in the OSCC tissues (r = - 0. 412,P < 0. 05). Conclusions The transcription factor Twist is up-regulated and E-cadherin is down-regulated,both of them play a critical role in the occurrence and malignant progression of OSCC.%目的 观察口腔鳞状细胞癌(OSCC)组织中转录因子Twist及上皮细胞钙黏蛋白(E-cadherin)的表达变化,并探讨其意义.方法 采用免疫组化SP法检测65例OSCC组织、45例正常口腔黏膜组织中Twist和E-cadher-in蛋白表达,分析Twist、E-cadherin表达与OSCC患者临床病理特征的关系,并采用Spearman等级相关分析法分析OSCC组织中Twist和E-cadherin表达的关系.结果 Twist在OSCC组织和正常口腔黏膜组织中的阳性表达率分别为72.31%(47/65)、11.11%(5/45),差异有统计学意义(P<0.05);E-cadherin在OSCC组织和正常口腔黏膜组织中的阳性表达率分别为23.08%(15/65)、86.67%(39/45),差异有统计学意义(P<0.05).OSCC组织中,Twist和E-cadherin异常表达与患者淋巴结转移和肿瘤临床分期有关(P均<0.05),与患者性别、年龄、组织分化程度、生长类型无关(P均>0.05).OSCC组织中,Twist和E-cadherin表达呈负相关(r=-0.412,P<0.05).结论 OSCC癌组织中转录因子Twist表达上调,E-cadherin表达下调;二者在OSCC发生和恶性进展中发挥重要作用.
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