胃癌组织中IL-22BP、IL-22、STAT3的表达变化及意义

摘要

Objective To detect the expression of interleukin-22 (IL-22)binding protein (IL-22BP),IL-22,signal transducer and activator of transcription-3 (STAT3)in the tissues of patients with gastric cancer,and to explore its signifi-cance. Methods Using immunohistochemical SP method to stain 37 cases of gastric cancer tissues and their matched nor-mal gastric tissues which were obtained from the proximal margin of the resection and beyond 5 cm to the gastric cancer sec-tion,the expression of IL-22BP,IL-22,and STAT3 was detected,and the relationships of the three parameters with clini-copathological features of gastric cancer were analyzed. Results The positive expression rates of IL-22BP in the gastric cancer tissues and the normal gastric tissues were 67. 6% (25 / 37)and 18. 9% (7 / 37);the positive expression rates of IL-22 in the gastric cancer tissues and the normal gastric tissues were 73. 0% (27 / 37)and 18. 9% (7 / 37);the positive expression rates of STAT3 in the gastric cancer tissues and the normal gastric tissues were 75. 8% (28 / 37)and 24. 3%(9 / 37),respectively. Significant differences were found in the positive expression rates of the three parameters between the gastric cancer tissues and the normal gastric tissues (all P < 0. 01). The positive expression of IL-22BP,IL-22,and STAT3 in the gastric cancer tissues was significantly related with the clinical stage,depth of invasion,and lymph node me-tastasis of the patients with gastric cancer (all P < 0. 05),but not related with gender and age (all P > 0. 05). In the gas-tric cancer tissues,the expression of IL-22BP was positively correlated with the expression of IL-22,and STAT3 (r =0. 526,0. 383,respectively;all P < 0. 05),and the expression of IL-22 was positively correlated with the expression of STAT3 (r = 0. 342,P < 0. 05). Conclusion IL-22BP,IL-22,and STAT3 are highly expressed in the gastric cancer tis-sues,the expression of them is related with the invasion and metastasis of gastric cancer,and the results suggest that they may synergistically promote the occurrence and development of gastric cancer.%目的 观察白细胞介素22结合蛋白(IL-22BP)、IL-22、信号传导和转录激活因子3(STAT3)在胃癌组织中的表达变化,并探讨其意义.方法 采用免疫组化SP法对经手术后的37例份胃癌组织及其配对的距癌旁5 cm外的正常胃组织标本37例份进行染色,检测IL-22BP、IL-22、STAT3表达,并分析三者与胃癌患者临床病理特征的关系.结果 在胃癌组织、正常胃组织中IL-22BP阳性表达率分别为67.6%(25/37)、18.9%(7/37),IL-22阳性表达率分别为73.0%(27/37)、18.9%(7/37),STAT3阳性表达率分别为75.8%(28/37)、24.3%(9/37),胃癌组织、正常胃组织中IL-22BP、IL-22、STAT3表达差异均有统计学意义(P均<0.01).IL-22BP、IL-22、STAT3在胃癌组织中的阳性表达与患者临床分期、浸润深度、淋巴结转移均有关(P均<0.05),与患者的性别和年龄无关(P均>0.05);胃癌组织中IL-22BP表达与IL-22、STAT3表达呈正相关(r分别为0.526、0.383,P均<0.05);IL-22表达与STAT3表达呈正相关(r=0.342,P<0.05).结论 胃癌组织中IL-22BP、IL-22、STAT3呈高表达,且三者表达与胃癌的侵袭和转移有关,三者可能协同促进胃癌的发生发展.