转染pcDNA-FLAG-EXOSC2质粒对宫颈癌HeLa细胞增殖与凋亡的影响

摘要

Objective To observe the effects of pcDNA-FLAG-EXOSC2 transfection on the proliferation and apoptosis of cervical cancer HeLa cells.Methods The pcDNA-FLAG-EXOSC2 recombinant plasmid was constructed.HeLa cells were transfected with pcDNA-FLAG-EXOSC2 plasmid (FLAG-EXOSC2 over-expression group) or pcDNA3.1 (+) plasmid (vector control group).Then, CCK-8 assay was performed to observe the cell proliferation of each group (the results were shown as OD450), while flow cytometry was utilized to measure the apoptotic rate.Results The OD450 values of the FLAG-EXOSC2 over-expression group and vector control group were 0.6652±0.05094 and 0.4436±0.03519 (both P<0.05).The apoptotic rates of the FLAG-EXOSC2 over-expression group and vector control group were 5.99%±0.37% and 7.57%±0.64%, respectively (both P<0.05).Conclusion Transfection of pcDNA-FLAG-EXOSC2 plasmid may significantly accelerate the proliferation and inhibit the apoptosis of HeLa cells.%目的 观察转染pcDNA-FLAG-EXOSC2质粒对宫颈癌HeLa细胞增殖与凋亡的影响.方法 构建pcDNA-FLAG-EXOSC2重组质粒.将体外培养的HeLa细胞分为EXOSC2过表达组、空质粒组,分别转染pcDNA-FLAG-EXOSC2重组质粒、pcDNA3.1(+)质粒.采用CCK-8法观察各组细胞增殖情况(结果以OD450表示),采用流式细胞术检测各组细胞凋亡率.结果 EXOSC2过表达组、空质粒组OD450分别为0.665 2±0.050 94、0.443 6±0.035 19,两组OD450相比,P<0.05.EXOSC2过表达组、空质粒组细胞凋亡率分别为5.99%±0.37%、7.57%±0.64%,两组细胞凋亡率相比,P<0.05.结论 转染pcDNA-FLAG-EXOSC2可以促进宫颈癌HeLa细胞增殖并抑制其凋亡.