CYFIP1过表达的鼻咽癌细胞株CNE2增殖、迁移能力观察

摘要

Objective To investigate the changes in the proliferation and migration of nasopharyngeal carcinoma cells with over-expression of cytoplasmic FMRP interacting protein 1 (CYFIP1). Methods A human nasopharyngeal carcinoma cell line (CNE2) was infected with a lentiviral vector containing CYFIP1 or the empty vector, which were used as the experimental group and blank group. The uninfected CNE2 cells acted as the control group. Cell proliferation was evaluated by MTT (represented by OD value) after cell culture for 24, 48, 72 and 96 h, separately. In clone formation assays, the cell culture was terminated when clones in plate could be seen by naked eyes and clones were counted. Cell migration was analyzed by Transwell chamber and Scratch assays. The cell migration distance and the number of penetrating cells were calculated. Results No significant difference was found in the OD value at different cell-culture time among these three groups (P>0.05). The number of clones in the experimental group, blank group and control group were 414±29, 426±30 and 381±59, respectively (P>0.05). The cell migration distance of the experimental group, blank group and control group was (6.71±0.1), (9.82±0.12) and (9.98±0.1) mm, respectively. Compared with the blank group and control group, the experimental group had a shorter migration distance (all P<0.05). The penetrating cells of the experimental group, blank group and control group were 37.33±1.53, 62.67±5.03 and 62.13±4.36. The experimental group had less penetrating cells than those of the blank group and control group (all P<0.05). Conclusions The cell migration of nasopharyngeal carcinoma cell line CNE2 with over-expression of CYFIP1 is inhibited. Abnormal expression of CYFIP1 maybe participates in the invasion and development of nasopharyngeal carcinoma.%目的 观察胞质FMRP相互作用蛋白1(CYFIP1)过表达的鼻咽癌细胞株CNE2增殖、迁移能力变化.方法 将含CYFIP1基因片段和空载片段的慢病毒转染鼻咽癌细胞株CNE2,分别作为实验组和空载组;以不进行转染操作的CNE2细胞为对照组.分别于培养24、48、72、96 h采用MTT实验观察各组细胞增殖情况(以OD值表示).进行细胞克隆形成实验,待平板中出现肉眼可见克隆时,终止培养并计算克隆形成数.分别采用划痕实验和Transwell迁移实验观察细胞迁移能力,测量细胞迁移距离,记录穿膜细胞数.结果 三组不同培养时间OD值相比,P均>0.05.实验组、空载组和对照组克隆形成数分别为(414±29)、(426±30)、(381±59)个,三组相比,P均>0.05.实验组、空载组、对照组细胞相对迁移距离分别为(6.71±0.10)、(9.82±0.12)、(9.98±0.10)mm,实验组细胞迁移距离小于空载组和对照组(P均<0.05).实验组、空载组、对照组穿膜细胞数分别为(37.33±1.53)、(62.67±5.03)、(62.13±4.36)个,实验组穿膜细胞数少于空载组和对照组(P均<0.05).结论 CYFIP1过表达的鼻咽癌细胞株CNE2迁移能力受到抑制.CYFIP1表达异常可能与鼻咽癌的浸润、发展有关.