大肠癌石蜡包埋组织中MiR-21表达变化及意义

摘要

目的 观察大肠癌石蜡包埋组织中微小RNA-21(miR-21)的表达变化,并探讨其临床意义.方法 从40例大肠癌患者石蜡包埋的癌组织和癌旁5 cm范围外的正常黏膜组织中抽提RNA,采用反转录实时荧光定量PCR法检测miR-21,并分析癌组织中miR-21表达与大肠癌患者临床病理特征的关系.结果 miR-21在癌组织中相对表达量为-4.28±1.67,在癌旁正常组织为-2.05±1.85,miR-21在癌组织中的相对表达量较癌旁正常黏膜组织升高(P＜0.05).在不同性别、肿瘤形态及发病部位患者的癌组织中miR-21相对表达量比较差异无统计学意义(P均＞0.05),在年龄大于中位年龄、存在淋巴结转移、分化程度低及已浸透浆膜层患者的癌组织组中miR-21相对表达量高于年龄小于中位年龄、无淋巴结转移、分化程度高及未浸透浆膜层患者的癌组织(P均＜0.05).结论 miR-21在大肠癌石蜡包埋组织中表达升高,其可能参与肿瘤的发生发展、分化及转移等生物学行为的调节;石蜡包埋组织可能成为研究肿瘤微小RNA的一种重要资源.%Objective To observe the expression changes of microRNA-21 (miR-21) in paraffin-embedded tissues of colorectal cancer,and to explore its clinical significance.Methods We extracted RNA from 40 cases of colorectal cancer samples and their corresponding cancer-adjacent normal tissues which were fixed by formalin and embedded by paraffin.MiR-21 expression was detected by reverse transcription quantitative real-time PCR,and we analyzed the relationship between the miR-21 expression and clinicopathologic features of colorectal cancer patients.Results The relative expression of miR-21 in the cancer tissues was-4.28±1.67,which was 4.69 times higher than that (-2.05±1.85) in the normal tissues adjacent to carcinoma (P＜0.05).There was no significant difference in the expression of miR-21 among patients with different sex,tumor shape and position (all P＞0.05).However,the level of miR-21 in patients with median age or higher,lymph node metastasis,low degree of differentiation and high infiltration depth of carcinoma was higher than that in patients with age lower than the median age,no lymph node metastasis,high degree of differentiation and low infiltration depth of carcinoma (all P＜0.05).Conclusions MiR-21 expression is increased in paraffin-embedded tissues of colorectal cancer,indicating miR-21 may be involved in the regulation of biological behaviors,such as the occurrence,development,differentiation and metastasis of colorectal cancer.Paraffin-embedded tissue may be an important resource for studying tumor microRNAs.