FGF4在肺癌组织中的表达及其对肺癌细胞增殖、转移的影响

摘要

Objective To observe the expression of fibroblast growth factor 4 (FGF4) in the lung cancer tissues,and to discuss the effects of FGF4 on the proliferation and metastasis of lung cancer cells. Methods We selected 40 cases of lung cancer tissues and their corresponding paracancerous tissues,human lung fibroblast cell line HFL1,human low metastatic lung cancer cell line NL9980,and human high metastatic lung cancer cell line A549. The mRNA expression of FGF4 in the tissues and cells was detected by RT-PCR,and the protein expression of FGF4 in the cells was detected by Western blotting. NlL9980 cells were transfected with Lipo2000 to construct stable cell line NL9980-FGF4-OE with over-expression of FGF4. The negative controls were NL9980 cell line only transfected with pCDNA3.1,that was NL9980-NC. A549 cells were transfected with Lipo2000 to construct the stable cell line A549-FGF4-si with knockout of FGF4. The negative control was A549 cell line transfected with pCDNA3.1,namely A549-NC. Meanwhile,the proliferation ability of NL9980-NC, NL9980-FGF4-oe,A549-NC,and A549-FGF4-si cells was detected by MTT assay,and the cell migration ability was detected by Scratch test. Results The mRNA expression of FGF4 in the lung cancer tissues was higher than that of the adjacent tissues (P < 0.05). What's more,the mRNA and protein expression levels of FGF4 was in the following order: A549> NL9980 > HFL1 (all P < 0.05). The proliferation capacity of NL9980-FGF4-oe cells was higher than that of NL9980-NC,at the same time,the proliferation capacity of A549-FGF4-si cells was lower than that of A549-NC cells (both P <0.05). The migration distance of NL9980-FGF4-oe cells was greater than that of NL9980-NC cells,meanwhile,the migration distance of A549-FGF4-si cells was shorter than A549-NC (both P < 0.05). Conclusion FGF4 is highly expressed in the lung cancer tissues,and its over-expression can promote the proliferation and metastasis of lung cancer cells.%目的 观察肺癌组织中成纤维细胞生长因子4(FGF4)的表达水平,探讨FGF4对肺癌细胞增殖和转移的影响.方法 取40例肺癌组织及其对应的癌旁组织,人高转移肺癌细胞A549、人低转移肺癌细胞NL9980、人肺成纤维细胞HFL1,用RT-PCR法检测组织和细胞中的FGF4 mRNA,Western blotting法检测细胞中的FGF4蛋白表达.取NL9980细胞,采用Lipo2000转染构建过表达FGF4的稳定细胞株NL9980-FGF4-oe,阴性对照为只转染pCDNA3.1的NL9980细胞(NL9980-NC);取A549细胞,采用Lipo2000转染构建敲除FGF4的稳定细胞株A549-FGF4-si,阴性对照为只转染pCDNA3.1的A549细胞(A549-NC).采用MTT法检测NL9980-NC、NL9980-FGF4-oe、A549-NC、A549-FGF4-si细胞的增殖能力,细胞划痕实验观察细胞迁移能力.结果 肺癌组织中FGF4 mRNA表达水平高于癌旁组织(P<0.05).FGF4 mRNA和蛋白表达水平A549>NL9980>HFL1(P均<0.05).NL9980-FGF4-oe细胞的增殖能力高于NL9980-NC,A549-FGF4-si细胞的增殖能力低于A549-NC细胞(P均<0.05).NL9980-FGF4-oe细胞的迁移距离大于NL9980-NC细胞,A549-FGF4-si细胞的迁移距离小于A549-NC细胞(P均<0.05).结论 FGF4在肺癌组织中表达升高,其过表达对肺癌细胞增殖及转移具有促进作用.