Eight Male Sterile Lines of Afalfal and three afalfal materials with better characters were get by Backcrossing that used as female and male parent,and carried hybrid seed production test in open pollination and artificial pollination. The results showed: (1) Megachile pollination could Effectively improve male sterile lines of alfalfa seed yield (33.66% ) ,and the per plant seed yield of No. 2 was the highest (3.16 g/plant). (2) Correlation and regression analysis indicated that branches of per plant was the most important factor which influenced the male sterile lines seed yield of alfalfa, and the setting percentage was the secondly.%以回交转育得到8个苜蓿雄性不育株(系)和3个优良苜蓿品种为亲本,分别在开放授粉和人工控制下进行杂交制种试验.结果表明:切叶蜂辅助授粉方式可以有效提高苜蓿雄性不育株(系)的制种产量.综合2种授粉方式下的制种产量,其中2号不育株(系)单株种子产量最高(3.16g/株);通过苜蓿雄性不育株(系)种子产量的相关分析和回归分析,明确了每株生殖枝数对不育株(系)单株种子产量作用影响最大,其次是结实率.
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