Immobilization of pegylated AMP on hydroxyapatite nanorods for antibiosis

摘要

Infection is one of the major reasons whichinduce failure of percutaneous Ti implants.Covalent bonding of antimicrobial peptides(AMPs)on Ti surfaces can avoid AMPs'blast release by physical absorption,but the reduced mobility affects their antibacterial efficiency.In this paper,HHC36 was pegylated with PEG12 and immobilized on hydroxyapatite(HA)nanorods to endow Ti surface with antibacterial activity and improved cytocom-patibility simultaneously.The obtained results indicated that HA nanorods immobilized with HHC36 showed antibacterial activity,and pegylation obviously increased the antibacterial activity of immobilized HHC36 against staphylococcus aureus.They reached 99.5%and 97.0%in vitro and in vivo,respectively.PEG12 is flexible,and it increases mobility of HHC36 and enables lateral diffusion of peptide molecules into the lipid double layer of microbial membrane,resulting in highly antibacterial activity of HHC36.Furthermore,HA nanorods immobilized with pegylated HHC36 have reduced inflammatory response in an infection model and should be potentially applied on percutaneous Ti implants for reducing infection-induced failure and enhancing biointegration.