神经调节蛋白1对体外循环心脏停搏大鼠的心肌保护作用研究

摘要

目的：探讨神经调节蛋白1（NRG-1）对体外循环（CPB）心脏停搏大鼠的心肌保护作用。方法选取成年健康雄性 SD 大鼠30只，随机分为正常对照组、模型组、NRG-1预处理组，每组10只。B、C 组大鼠参照文献建立 CPB 模型，NRG-1预处理组大鼠在 CPB 心脏停搏前30 min 腹腔注射NRG-1，正常对照组大鼠置于恒温干净环境中正常喂养。比较3组大鼠血清肌酸激酶同工酶（ CK-MB）、乳酸脱氢酶（ LDH）、丙二醛（ MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）水平及血浆内皮素（ET）、血栓素 A2（TXA2）、前列环素（PGI2）水平。结果模型组和NRG-1预处理组大鼠血清CK-MB、LDH、MDA 水平及血浆 ET、TXA2水平高于正常对照组，血清 SOD、GSH-Px水平及血浆 PGI2水平低于正常对照组（P ﹤0.05）；NRG-1预处理组大鼠血清CK-MB、LDH、MDA 水平及血浆ET、TXA2水平及低于模型组，血清 SOD、GSH-Px水平及血浆 PGI2水平高于模型组（P ﹤0.05）。结论 CPB 可引起心脏停搏大鼠心肌损伤，NRG-1对 CPB 心脏停搏大鼠具有一定心肌保护作用。%Objective To investigate the myocardial protective effect of neuregulin 1(NRG-1)in rats with cardiac arrest under cardiopulmonary bypass(CPB). Methods A total of 30 healthy adult male SD rats were selected and randomly divided into A group,B group and C group,each of 10 rats. Of B group and C group,CPB model was prepared according to the reported literatures,meanwhile rats of C group were given intraperitoneal injection of NRG-1 before 30 minutes of cardiac arrest;rats of A group were normally fed in constant temperature and clean environment. Serum levels of CK-MB,LDH,MDA, SOD and GSH-Px,plasma levels of ET,TXA2 and PGI2 were compared among the three groups. Results Serum levels of CK-MB,LDH and MDA,plasma levels of ET and TXA2 of B group and C group were statistically significantly higher than those of A group,while serum levels of SOD and GSH-Px,plasma PGI2 level of B group and C group were statistically significantly lower than those of A group(P ﹤ 0. 05);serum levels of CK-MB,LDH and MDA,plasma levels of ET and TXA2 of C group were statistically significantly lower than those of B group,while serum levels of SOD and GSH-Px,plasma PGI2 level of C group were statistically significantly lower than those of B group(P ﹤ 0. 05). Conclusion CPB may cause myocardial damage in rats with cardiac arrest,while NRG-1 has certain myocardial protective effect in rats with cardiac arrest under CPB.