二斑叶螨对螺螨酯抗药性及对18种杀螨剂交互抗性

摘要

The S strain of Tetranychus urticae Koch collected from Xinglong Mountain in Lanzhou of Gansu were reared by amphoterotoky.The SP-R strain was selected by spirodiclofen.Both strains were tested by slide dip bioassay method.The results showed that resistance development was slow at the beginning, stable at mid-term stage but fast at later period.After insecticide resistance was selected for 26 generations, the RI was 58.83.The SP-R had obvious cross resistance to fenpropathrin and cyhalothrin (resistance index, RI＞5.00).But the SP-R had no significant cross-resistance to fenbutatin oxide, clofentezine, celastrusangulatus, avermectins, cypermethrin, pyrclofentezi, pyr-isocarbophos, azocyclotin, dicofol, pyridaben and omethoate (RI＜5.00), and cross-resistance to liuyangmycin, chlorpyrifos, hexythiazox, die-clofentezi and fenpyroximate were negative and RI were less than 1.%采自甘肃兰州兴隆山公园的二斑叶螨(Tetranychus urticae Koch),用雌雄单系培养敏感品系(S),用螺螨酯处理二斑叶螨种群培养抗性品系(SP-R),用室内生测法对二斑叶螨S和SP-R品系进行室内毒力测定.结果表明,二斑叶螨对螺螨酯抗性发展初期较慢,中期稳定,后期较快,选育至26代抗性指数(RI)达58.83.SP-R对甲氰菊酯、氯氰菊酯有一定的交互抗性,RI分别为11.54和10.03;对苯丁锡、四螨嗪、苦皮藤生物碱、阿维菌素、氯氟氰菊酯、哒螨·四螨嗪、哒·水胺硫磷、三唑锡、三氯杀螨醇、哒螨灵、氧化乐果无交互抗性(1＜RI＜5.00);对浏阳霉素、毒死蜱、噻螨酮、柴油、哒螨灵、唑螨酯可能存在负交互抗性(RI＜1).