By adopting improved CAS methods, the strains antagonistic against plant pathogens were firstly screened from the 16 PGPR strains, and the antagonistic experiments were then conducted by using 5 PGPR strains to 3 types of plant pathogens using plate cultivation.The results showed that LHS11 had the best inhibitory effects on Fusarium oxysporum f.sp.cucumerinum and F.oxysporum f.sp.niveum, vith the inhibition ratio over 80%; the inhibition ratio of 191 against F.oxysporum f.sp.cucumerinum was 74%.The inhibitory effects of LHS11 and 191 on Rhizoctonia solani were similar ( 64% and 65%, respectively).Both 191 and LHS11 could be used as PGPR biocontrol agents with good inhibitory effects and high application potential.%采用改进的CAS定性、定量方法,从16株PGPR菌株中初步筛选出具有抗病原真菌作用的菌株,再利用平板对峙法将筛选出的5株PGPR菌与3种病原真菌进行拮抗试验.结果表明,LHSll对黄瓜枯萎病菌(Fusarium oxysporUTh f.sp.cucumerinum),西瓜枯萎病菌(Fusarium oxysporum f.sp.niveum)的抑制效果最好,抑菌率达到80%以上;其次是191对黄瓜枯萎病菌,抑菌率为74%,LHSll、191对立枯丝核菌(Rhizoctonia solani)的抑制效果相近,抑菌率分别为64%和65%.191和LHSll是抑菌效果较好的生防PGPR菌株,具有较好的应用潜力.
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