Effects of different concentrations of imidacloprid on the esterase activity of Rhopalosiphum padi were evaluated. Esterase activity and its kinetic parameters were estimated in R. Padi by using biochemical analysis, following treatment with sublethal and lethal concentrations of imidacloprid. The results showed that exposure of aphids to imidacloprid at two doses enhanced their enzyme activity and protein content compared with those of the control, while the protein content was significantly different in insects that received sublethal and lethal doses of insecticides. However, the Km value and Vmax value of esterase were not different between the two treated groups and the control. It suggested that the affinity of esterase to the substrate did not vary with the treatment doses, but significantly increased protein content of esterase in the insecticide-treated aphids.%采用生物化学方法研究不同剂量吡虫啉对禾谷缢管蚜酯酶活性和酶动力学参数的影响.结果表明,不同浓度吡虫啉处理禾谷缢管蚜后,可极显著诱导禾谷缢管蚜蛋白含量增加和酯酶活力增强,且不同处理组间蛋白含量差异显著,但不同剂量吡虫啉处理并未对禾谷缢管蚜酯酶动力学参数产生明显影响.不同浓度吡虫啉处理禾谷缢管蚜后,并未改变其酯酶的性质,但酯酶的表达量显著增加.
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