抗黄矮病小麦新品系的分子标记检测研究

摘要

本研究对以小麦-中间偃麦草异附加系L1和小麦-中间偃麦草部分双二倍体‘无芒中4’为抗源选育出的抗黄矮病小麦新品系进行分子检测和抗病性鉴定.通过应用RAPD、SSR、SCAR 3种分子标记OPF15、Xgwm37、SC-W37进行分子检测,并采用人工接种和大田自然感病的方式进行抗黄矮病鉴定,筛选到了‘93646’、‘2003-2’等高抗黄矮病的小麦新品系.分子检测抗黄矮病基因与田间抗病鉴定结果基本一致,应用的3种PCR标记都可以检测出抗病材料,但SCAR标记SC-W37特异性强、稳定性好,可在小麦抗黄矮病育种早代选择过程中发挥重要作用.%Some BYDV translocation lines were studied by using wheat- Thinopyrum intermedium the addition line L1 and a partial amphiploid 'Zhong 4 awnless' as the resistance parents. This translocation lines were used to screen molecular markers and identification of disease resistance. A RAPD marker OPF15, SSR marker Xgwm37 and SCAR marker SC-W37 were developed for the BYDV resistance. Some new strains with excellent BYDV resistance were selected by identification of disease resistance and molecular marker, including ' 2003-2' and '93646'. The results showed that the BYDV resistant materials could be detected by PCR markers. The SCAR marker SC-W37 had high specificity and good reliability, which might play an important role in early-generation selection in wheat breeding.