[Objective] The content of 2' ,3'-epoxyanisolactone in three different organ of Clausena dunniana were tested, the biochemical and metabolic effect on echinochloa crusgalli L. Were investigated. [MethodjThe content of 2',3'-epoxyanisolactone were analyzed by HPLC analyzing, bioassay of E. Crusgalli was tested by small glass method. [Result] The result indicated that there are 0.43% in flower, 3.21% in seeds and 3.33% in the leaf and bark, respectively. The result of assay on metabolism to E. Crusgalli indicated that 2',3'-epoxyanisolactone could cause the weakness of vigor and functions of root systems, and the decrease of total amount of protein of E. Crusgalli , 2,3-epoxyanisolactone could increase the quantity of amino acid, with the series of concentration of 2', 3'-epoxyanisolactone. 50 μg/Ml, 25 μg/Ml, 12.5 μg/Ml, 6.25 μg/Ml and 3.125 μg/Ml, the quantity of a-mino acid of controlled seeding were 1.402 8 mg/g, 1.317 2 mg/g, 1.250 2 mg/g, 1.128 7 mg/g and 0.903 2 mg/ g respectively, which was higher than the control at 0.832 1 mg/g. Protein quantity of controlled seeding of E. Crusgalli decrease 75.91%, 66.43%, 62.43%, 58.57% and 58.03% compared to the control respectively. [Con-clusion]The result indicated that 2',3'-epoxyanisolactone has influence on E. Crusgalli.%[目的]测定黄皮素内酯Ⅱ(2',3'-epoxyanisolactone)在黄皮[Clausena lansium(Lout.)Skeels]植株的花、枝叶和果核中的含量,明确其对稗草生化代谢的影响.[方法]采用液相色谱法进行含量的测定,小杯法培养供试稗草.[结果]黄皮素内酯Ⅱ在花粗提物中的含量较少,仅为0.43％,在枝叶和果核中的含量分别为3.33％和3.21％.黄皮素内酯Ⅱ处理稗草植株,处理后稗草根中蛋白含量和氨基酸含量与未经处理存在明显的差异,在50 μg/mL的浓度下,可以显著地降低稗草植株内的蛋白含量和增加植株内的氨基酸含量.当处理浓度为50、25、12.5、6.25、3.125 μg/mL时,样品中的含氮量分别为1.402 8、1.317 2、1.250 2、1.128 7、0.903 2 mg/g,比对照0.832 1 mg/g均有所增加.黄皮素内酯Ⅱ处理后,稗草的蛋白含量与对照相比有所下降,处理浓度为50、25、12.5、6.25、3.125 μg/mL时,蛋白含量比对照分别降低75.91％、66.43％、62.43％、58.57％和58.03％.[结论]黄皮素内酯Ⅱ对稗草代谢有一定影响.
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