为大量获得研究所需的小麦禾谷孢囊线虫2龄幼虫,在室内离体条件下研究了预处理条件、孵化温度、低温预处理时间以及田间采样时期对山东泰安地区小麦禾谷孢囊线虫幼虫孵出量的影响.结果表明:5℃低温预处理条件下,孢囊浸水处理有利于幼虫孵出；低温预处理后,孵化温度为15℃处理的2龄幼虫孵出率高于20℃处理的孵出率；低温预处理时间为8周的2龄幼虫孵出率最高；6月和8月田间采集的孢囊幼虫孵出率显著高于7月和9月,其中8月采集的孢囊幼虫孵出率最高,为最佳采集时间.%In order to obtain enough second stage juveniles of Heterodera avenue, the effects of the pretreatment conditions, emergence temperature, cyst pretreatment time and the time of field collecting on their emergence characteristics of Tai'an population of H. Avenae were studied in vitro. The results indicated that treatment by dipping cysts in water could benefit juvenile emergence at 5 ℃. For the pretreated cysts at 5 ℃, there was significant increase in juvenile emergence rate at 15 ℃ as compared to 20 ℃. The cysts pretreated at 5 °C for eight weeks had the highest emergence rate. The juvenile emergence rate of the cysts collected from fields in June and August was significantly higher than that of the cysts collected in July and September, but August was the best filed collecting period.
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