福建及贵州等地烟草青枯菌系统发育分析

摘要

The phylogeny of tobacco Ralstonia solanacearum strains from Fujian and Guizhou was studied. [Method] Sixty-two Chinese R. Solanacearum strains isolated from tobacco were analyzed using the phylotype classification schemes. [Result] Phylogenetic analysis of endoglucanase gene sequences showed that all isolates belonged to phylotype I, including 4 different sequevars: 15, 17, 34 and 44. All of the 34 sequevar strains were collected from Fujian Province. Only 3 strains were identified as sequevar 44. Alighment analysis of amino acid sequences deduced from avrA gene could not distinguished Chinese R. Solanacearum strains from the reference strain RS1000. [Conclusion] R. Solanacearum strains on tobacco from Fujian and Guizhou had genetic differentiation to some degrees.%[目的]探寻烟草上青枯菌的系统发育.[方法]采用演化型分类框架对福建及贵州等地的62个烟草青枯病菌株进行鉴定分析.[结果]基于内切葡聚糖酶基因系统发育学的分析结果表明:所有参试菌株均归属于青枯菌亚洲分支的4个序列变种,分别为序列变种15、17、34和44；尚未发现归属于美洲或非洲分支的烟草青枯病菌株.其中序列变种15和17为优势菌系,序列变种34的菌株都来自福建省,只发现3个菌株属于序列变种44.基于avrA基因的氨基酸序列比对结果表明4个序列变种的avrA基因都属于RS1000类型.[结论]本研究表明福建及贵州等地烟草上的青枯菌存在一定的遗传分化.