一种改良的快速提取小型昆虫总RNA的方法

摘要

The silica method,a simple and efficient protocol,is broadly used in plant RNA extraction.In this study,the method was modified and used in small insects,such as Myzus persicae,Bemisia tabaci Q sibling species and Frankliniella occidentalis.Meantime,we compared the RNA extraction efficiency of small insects with the modified silica protocol,the modified one-step procedure and Trizol method.The results showed that the highest quality and yields of RNA were obtained by modified silica RNA extraction protocol.This protocol has the advantages of time-saving,lower cost as well as easy-to-handle and can be used for RNA extraction of insects.%硅粒吸附法是一种常用的植物总RNA提取方法,本文对其进行改良后应用于小型昆虫总RNA提取,同时与小型昆虫RNA提取常用的改进的一步法和Trizol法进行了比较.试验结果表明:改良的硅粒吸附法是一种经济高效的小型昆虫总RNA提取方法,RNA产率高,纯度佳.同时,整个试验操作步骤简单、省时(约1 h),所用试剂普遍、廉价.