对白头翁叶斑病菌胞壁结合黑色素和胞外黑色素进行了理化性质和红外光谱扫描测定，结果表明两者具有相似的理化性质，均易溶于KOH、H2 O2和NaClO，不溶于水、乙醇和丙酮。红外光谱分析表明，白头翁叶斑病菌YS-24菌株的胞壁结合黑色素与胞外黑色素为同一种类型的黑色素。DHN黑色素的特异性抑制剂—三环唑，对白头翁叶斑病菌黑色素的产生有明显的抑制作用；以白头翁叶斑病菌基因组DNA为模板，通过PCR扩增，得到了聚酮体合成酶基因的同源片段AaPKS，初步推断白头翁叶斑病菌黑色素合成属于DHN途径。%The characteristics of melanin,extracted from cell wall and fermented filtrate of Ascochyta anemones, were determined by diagnostic test and infrared spectroscopy. The results indicated that they have similar physical and chemical properties,and they are soluble in KOH,H2 O2 and NaClO,but not in water,ethanol and acetone. Infrared spectrum analysis showed that the wall-bound melanin and extracellular melanin belonged to the same type. Tricyclazole,a specific inhibitor of DHN melanin synthesis,could inhibit the fungal melanin’s biosynthesis of A.anemones. The homologous fragment of polyketide synthase gene (AaPKS)was amplified by PCR using A.anemones genomic DNA as a template. The results suggested that the melanin in A.anemones might be synthe-sized from DHN pathway.
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