杜仲内生真菌中抗玉米纹枯病活性菌株的筛选

摘要

In this study,thirty-two strains of endophytic fungi were isolated from the leaves,stems and fruits of Eucommia ulmoides. And the antimicrobial activity of endophytic fungi was examined against maize Rhizoctonia solani using confront antibiotic culture experiment,growth inhibition measurements,disease-control pot experi-ment. The results indicated the strain DZGS08 had strong inhibit against maize Rhizoctonia solani with more than 10 mm inhibition zone,and the fermentation broth of DZGS08 had a strong inhibitory effect on the pathogenic fungi,its inhibitory rates on Rhizoctoniasolani;was 50.44% ,Moreover,the control efficacy of the fermentation broth of the DZGS08 was 34.03% by pot experiment. It was revealed microscopically the strain DZGS08 could cause the abnormity and tortuosity of mycelial of phytopathogen and so on. Then,the strain DZGS08 was identi-fied of molecular,the results showed that the strain DZGS08 belonged to Thielavia.%对杜仲植物中的内生真菌进行分离纯化，共得到32株菌株。以玉米纹枯病菌为指示菌，对杜仲内生真菌进行对峙培养法、菌丝生长速率法以及盆栽控病试验等研究。研究结果显示，内生真菌DZGS08对玉米纹枯病菌具有较好的拮抗作用，抑菌带宽度大于10 mm；其菌丝生长速率试验显示DZGS08的发酵液抑菌率达到50．44％；且玉米盆栽试验结果表明，菌株DZGS08发酵液对玉米纹枯病的防治效果为34．03％。显微观察结果表明，拮抗菌株DZGS08能造成病原菌菌丝扭曲、畸形等现象。随后，对该菌株的ITS 序列进行测定分析，序列分析和聚类结果表明该菌株为梭孢壳属（Thielavia）的真菌。