354份小麦种质中抗白粉病基因Pm21和Pm13的分布研究

摘要

为了解本课题组现有种质资源中抗白粉病基因 Pm21和 Pm13的分布情况,本研究利用共分离分子标记SCAR 1400和 SCAR 564,对收集于全国多个育种单位的354份小麦种质进行检测。结果表明：在检测的354份种质中,携带 Pm21的有21份,分别为‘石 H083-366’、‘BH50890-1-1’、‘06Y06’、‘南农02Y393’、‘09P80’、‘09P131’、‘09P283’、‘黔麦18号’、‘黔早麦15-3’、‘黔979-1’、‘黔9963-3’、‘黔9961-2’、‘YN06’、‘绵杂麦168’、‘MR168’、‘09品 A6’、‘09品 E6’、‘内麦836’、‘内麦9号’、‘绵阳39’和‘绵麦367’,检出率为5.9%,其中,8份来源于四川,来源于江苏和贵州的各有5份,河北、陕西和云南的材料中各有1份；仅有1份种质‘中大01’携带 Pm13,来源于河南,检出率为0.28%。本研究结果可为抗白粉病基因资源的有效利用提供重要参考依据。%To understand the distribution of powdery mildew resistance gene Pm 21 and Pm 13 among 354 wheat germplasm resources collected from many breeding units all over the country and kept in wheat research center of Henan Institute of Science and Technology,their co-segregated molecular markers SCAR 1 400 and SCAR 5 64 were used to assist detection in this study.The results showed that 21 wheat germplasms among the 354 germplasm re-sources were confirmed to carry the powdery mildew resistance gene Pm 21,including ‘Shi H083-366’,‘BH50890-1-1’,‘06Y06’,‘Nannong 02Y393’,‘09P80’,‘09P131’,‘09P283’,‘Qianmai 18’,‘Qianzaomai 1 5-3’,‘Qian 979-1’,‘Qian 9963-3’,‘Qian 9961-2’,‘YN06’,‘Mianzamai 1 68’,‘MR1 68’,‘09 pin A6’,‘09 pin E6’,‘Nei-mai 836’,‘Neimai 9’,‘Mianyang 39’and ‘Mianmai 367’.The positive rate was 5.9%.Among these Pm 21-pos-itive germplasms,eight were collected from Sichuan Province,five from Guizhou and Jiangsu,and one from He-bei,Shanxi and Yunnan.Additionally,only one germplasm named ‘Zhongda 01’was confirmed to carry Pm 13, which was collected from Henan Province.The Pm 13-positive rate was 0.28%.The results of this study may pro-vide important information for effective utilization of the powdery mildew resistance gene resources.