为了明确小麦条锈菌毒性、遗传多样性以及温度敏感性三者之间的关系，本研究利用21个已知抗条锈病基因近等基因品系对2010－2011年生长季采自6个省市78株已知温度敏感性（ET50）的小麦条锈菌群体进行毒性鉴定，并利用 AFLP 技术对其进行遗传多样性分析。苗期毒性鉴定结果表明，不同省市小麦条锈菌群体的毒性基因多样性存在一定差异，甘肃菌株群体毒性多样性指数 H 值最高，为0．2693，云南菌株群体最低，为0．1504。遗传多样性结果显示，6省市小麦条锈菌群体遗传多样性指数 H 值范围在0．1255～0．1653之间，遗传一致度 GI 为0．9647～0．9872，遗传距离 GD 为0．0129～0．0360。三者的相关性分析表明，条锈菌群体毒性多样性与平均ET50显著负相关，与 ET50变异系数正相关，而与遗传多样性没有显著的相关关系。%To define the relationships among virulence,genetic diversity and temperature sensitivity of Puccinia striiformis f.sp.tritici isolates,78 isolates collected from 6 provinces during growing seasons in 2010-2011 with known ET50 values were analyzed for virulence and genetic diversity by using 21 differential cultivars and AFLP technique,respectively.The result of virulence diversity at seedling stage indicated that the Nei’s gene diversity (H)of virulence in Gansu was the highest (0.269 3),and the lowest was in Yunnan (0.150 4).The genetic diver-sity result showed that the Nei’s gene diversity (H)was between 0.125 5 and 0.165 3,the genetic identity (GI) was between 0.964 7 and 0.987 2,and the genetic distance (GD)was between 0.012 9 and 0.036 0.The correlation analysis showed that virulence diversity was significantly and negatively correlated with the average value of ET50 ,and positively correlated with the coefficient of ET50 variation,but not significantly correlated with genetic diversity.
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