为了解甘肃省小麦孢囊线虫病发生程度和分布情况，2009－2012年于小麦抽穗至灌浆期，采用 Z 字形取样法，取根际0～20 cm 土壤，用漂浮法分离孢囊，统计100 g 土样的孢囊数量，用形态学方法鉴定线虫。从甘肃省12个地区50个县区，134个乡镇共采集到463个小麦田间土样。结果表明，孢囊平均检出率为47．9％，其中冬麦区孢囊平均检出率为61．5％，春麦区平均检出率为34．2％，冬麦和春麦混播区为52．3％，冬麦、春麦及冬春麦混播区平均孢囊数分别为3．5、6．8和3．9个／100 g 土样。其中平凉市灵台县和兰州市永登县孢囊线虫发生最为严重，个别地块每100 g 土样中孢囊数超过40个。根据孢囊形态学特征的观察，将甘肃省采集到的孢囊线虫鉴定为禾谷孢囊线虫（Heterodera avenae ）。研究结果可为有效防治甘肃省麦类孢囊线虫病提供依据。%In 2009-2012,soil samples in depth of 0 20 cm were collected by zigzag sampling from heading to filling stage of wheat.Cysts of cereal cyst nematode (CCN)were separated using floating method and counted per 100 g soil,and then identified by morphological characteristics.Four hundred and sixty-three soil samples covered 50 counties in 12 regions of Gansu Province were collected in the systematic investigation.The results showed that the total incidence of cyst nematodes of wheat was 47.9%.And the incidence of cyst nematodes in winter wheat, spring wheat and mixed winter wheat and spring wheat region were 61.5%,34.2% and 52.3%,respectively.The average density of cysts in winter wheat,spring wheat and from mixed winter wheat and spring wheat region were 3.5 cysts/100 g soil,6.8 cysts/100 g soil and 3.9 cysts/100 g soil,respectively.The highest cyst density occurred in Lingtai County of Pingliang City and Yongdeng County of Lanzhou City with more than 40 cysts/100 g soil.All the cyst nematodes of wheat in Gansu were identified as Heterodera avenae based on the morphological character-istics.This work can provide a basis for effective prevention of wheat cyst nematode in Gansu Province.
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