西瓜嗜酸菌趋化性的初步研究

摘要

瓜类细菌性果斑病是世界范围的检疫性细菌病害，病原菌为西瓜嗜酸菌，带菌种子为主要侵染源。病原细菌在寄主表面的定殖能力与其致病能力关系密切，而趋化性是决定定殖能力的关键因素之一，研究不同物质对西瓜嗜酸菌趋化性的影响对防治瓜类细菌性果斑病具有重要意义。本文采用毛细管法，研究了碳源、氨基酸、有机酸及其他物质对西瓜嗜酸菌趋化性的影响。结果表明，所测碳源中，麦芽糖、葡萄糖、乳糖、蔗糖和半乳糖均显著促进西瓜嗜酸菌的趋化性；所测氨基酸中，L-精氨酸、L-天冬氨酸、L-组氨酸、L-谷氨酸、L-亮氨酸、L-缬氨酸、L-谷氨酰胺和 L-丙氨酸显著促进西瓜嗜酸菌的趋化性；所测有机酸中，琥珀酸、半乳糖醛酸和酒石酸显著促进西瓜嗜酸菌的趋化性；氯化钠、硫酸镁等对西瓜嗜酸菌的趋化性无显著影响。%Bacterial fruit blotch (BFB)is a worldwide quarantine disease,caused by Acidovorax citrulli ,and seeds carrying bacteria are the main source of infection.The colonization of pathogenic bacteria in hosts is highly relat-ed to virulence,and chemotaxis is a key factor to the colonization of A.citrulli .Studying the effect of different substances on the chemotaxis of A.citrulli played a great role in preventing BFB.In this study,capillary method was used to determine the effects of carbon,amino acids,and organic acids on the chemotaxis of A.citrulli .The results showed that,among the carbon resources,maltose,glucose,lactose,sucrose,and galactose significantly promoted the chemotaxis of A.citrulli ;among the amino acids,L-arginine,L-aspartic acid,L-histidine,L-glu-tamic acid,L-leucine,L-valine,L-glutamine and L-alanine significantly promoted its chemotaxis;among the or-ganic acids,succinic acid,galacturonic acid and tartaric acid significantly promoted chemotaxis of A.citrulli ;oth-er substances such as NaCl and MgSO4 had no significant effect on its chemotaxis.