内蒙古和黑龙江的核盘菌菌丝融合群分化及致病性研究

摘要

To identify the genetic diversity of Sclerotinia sclerotiorum ,44 isolates from Inner Mongolia and Hei-longjiang were ascertained by mycelial compatibility groups (MCGs)screening methods,and the differences among MCGs were observed by comparing their differences in mycelia growth rate,pathogenicity and production of oxalic acid and total acids.The results showed that the 44 isolates were grouped in 25 MCGs,among which 14 MCGs consisted of only one isolate,with a proportion of 56.0%.Significant differences were found in mycelia growth rate,pathogenicity and production of oxalic acid and total acids within and among MCGs (P <0.001)re-gardless of their geographic origins.Correlation analysis indicated that S .sclerotiorum pathogenicity was positive-ly related to oxalic acid production(r =0.484,P ≤0.01)and negatively related to pH(r =-0.580,P ≤0.01), but not related to the mycelia growth rate.There was a negative relationship between pH values and oxalic acid production(r =-0.392,P ≤0.01),suggesting that oxalic acid production contributed most to the total produc-tion of acids released by S .sclerotiorum .%为明确核盘菌的遗传多样性，对采自内蒙古和黑龙江不同地区的44株核盘菌进行了菌丝融合群确定，并比较了不同菌丝融合群间菌丝生长速度、致病力、草酸和总酸产量的差异。结果表明：供试44个菌株分为25个融合群，其中有14个融合群仅由单一菌株组成，所占比例为56．0％。菌丝融合群内和菌丝融合群间菌丝生长速度、致病力、草酸和总酸产量都表现出显著差异（P ＜0．001），并与菌株的地理来源无关。相关分析表明核盘菌菌株的致病力与菌株草酸产量呈正相关（r＝0．484，P ≤0．01），与 pH 呈负相关（r ＝－0．580，P ≤0．01），与菌株的生长速度无关；草酸产量与 pH 高低（表示总酸的分泌量）负相关（r＝－0．392，P ≤0．01），进一步表明核盘菌菌株产生的总酸中草酸量占了很大的比例。