ITS序列及其SNP位点在外来入侵杂草长芒苋、西部苋和糙果苋物种鉴定中的应用

摘要

Alien invasive weeds Amaranthus palmeri,A.rudis and A.tuberculatus are difficult to distinguish from each other,and taxonomical identification only depends on seed morphologies.In this study,A.palmeri,A.rudis and A.tuberculatus could be identified quickly and precisely through PCR-RFLP method,based on ITS and 26S rDNA analyses,and specific primers designed by SNP.The results show that there is no intraspecific variation within A.palmeri populations,but interspecific variation is significant.A.palmeri can be differentiated from other congeneric species by ITS.However,differences of ITS sequences between A.rudis and A.tuberculatus are small,they should be defined further by SNP analysis.%对于以种子形态为物种主要识别依据的口岸部门,外来入侵杂草长芒苋、西部苋和糙果苋一直是鉴定的难点.本文对34种苋属植物的ITS序列和26S rDNA进行分析,通过SNP变异位点及特异性引物,借助PCR-RFLP方法,对长芒苋、西部苋与糙果苋进行快速、准确的分类鉴定.ITS序列分析表明,长芒苋种内变异小,种间变异显著,可与其他苋属植物明显区分.西部苋与糙果苋之间ITS序列差异小,需依据SNP位点来区别.