杂草是菜豆金色花叶病毒属病毒的重要中间寄主,常富集多种该属病毒及其卫星病毒.2014年,在云南红河常见杂草苣荬菜Sonchus arvensis上出现了疑似菜豆金色花叶病毒属病毒病症状.利用克隆、测序和生物信息学分析技术对其所含病毒进行分离鉴定,结果从1株病样中共获得了两条菜豆金色花叶病毒属病毒全序列、两条β卫星全序列和一条α卫星全序列.序列分析显示,两条菜豆金色花叶病毒属病毒全序列与中国胜红蓟黄脉病毒相似性最高,分别为99％和96％,确定为中国胜红蓟黄脉病毒的分离物.两条β卫星全序列与赛葵黄脉β卫星相似性最高,为97％,确定为赛葵黄脉β卫星的一个分离物.α卫星全序列与中国番茄黄化曲叶α卫星相似性最高,为86.3％,是中国番茄黄化曲叶α卫星的一个分离物.这是菜豆金色花叶病毒属病毒病害复合体在中国侵染苣荬菜的首次报道.%Weeds are crucial intermediate hosts of begomoviruses and frequently harbor multiple begomoviruses and associated satellites.Sonchus arvensis,a common weed in Honghe,Yunnan Province,showed symptoms typical of begomovirus infection in 2014.Begomoviruses associated with S.arvensis was studied using cloning,sequencing and bioinformatic analysis.Two full-length begomoviruses,two betasatellites and one alphasatellite were isolated from one sample.Sequence analysis showed that these begomovirus sequences shared the highest identity (99％ or 96％) with Ageratum yellow vein China virus (AYVCNV),confirming both of them as an isolate of AYVCNV.These two betasatellite sequences shared 97％ identity with Malvastrum yellow vein betasatellite (MaYVB),confirming all of them as isolates of MaYVB.The alphasatellite sequence shared 86.3％ identity with Tomato yellow leaf curl China alphasatellite (TYLCCNA),confirming it as an isolate of TYLCCNA.This is the first report of begomovirus disease complex associated with S.arvensis in China.
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