油茶暹罗刺盘孢菌群体遗传结构分析

摘要

Colletotrichum siamense is one of the primary anthracnose pathogen of Camellia oleifera from most producing areas in China.To clarify the population genetic structure will provide theoretical basis for the effective control of plants anthracnose.The population genetic structure of 57 C.siamense strains collected from Hainan,Jiangxi,Hunan and Guangxi of China were analyzed in this paper.According to the assembled sequences of ITS,CAL and GAPDH genes,the 57 C.siamense strains were defined into 13 haplotypes,and the haplotype H7 was found dominant and distributed in all collection sites.There is large genetic differentiation between different geographical populations.AMOVA analysis showed that the genetic variation mainly took place within population,and the pathogen doesn't experience large-scale population expansion.The result showed that the natural population of C.siamense had rich genetic diversity.%暹罗刺盘孢菌是油茶炭疽病病原之一,在我国多个油茶产区均有分布.研究油荼暹罗刺盘孢菌群体遗传结构可为全面、有效防治油茶炭疽病害提供理论依据.本研究对分离自海南、江西、湖南、广西4省(自治区)6个地区暹罗刺盘孢菌菌株的ITS、CAL和GAPDH 3个基因的序列进行群体遗传结构分析.根据拼接的上述3个基因的序列,57个暹罗刺盘孢菌菌株可定义为13个单倍型,其中单倍型H7为主要单倍型,分布于本研究所涉及的所有地区.病菌不同地理种群间的遗传分化较大,AMOVA分析显示,遗传变异主要发生在种群内,病菌未经历过大规模的种群扩张.研究结果表明油茶炭疽病原暹罗刺盘孢菌种群具有丰富的遗传多样性.