辣椒轻斑驳病毒凤城分离物的鉴定、全基因测序及系统进化分析

摘要

辣椒轻斑驳病毒Pepper mild mottle virus (PMMoV)属于烟草花叶病毒属,是辣椒上重要的病原病毒之一,近年来PMMoV对辽宁部分辣椒产区造成严重危害.本文采用DASELISA检测以及RT-PCR的方法首次从辽宁省凤城市蔬菜产区辣椒病叶中检测出PMMoV,暂命名为PMMoV-FC.设计3对特异性引物对其进行扩增并测序后得到该病毒分离物全基因序列.将PMMoV-FC的全基因序列与已报道的国内外9个PMMoV分离物进行同源性分析,结果表明,其同源性介于94.3％～99.7％0之间.基于全基因序列的系统发育进化分析表明,PMMoV-FC与国内分离物、日本分离物以及美洲分离物亲缘关系密切,而与韩国和西班牙分离物亲缘关系稍远.鉴于该病毒在辣椒上造成的严重危害,对于PMMoV-FC在辽宁地区的发生以及防治仍需进行更为详细的研究.%Pepper mild mottle virus (PMMoV),a member of Tobamovirus,is a major viral pathogen of pepper.In recent years,PMMoV has caused great loss to pepper production in Liaoning Province.In this study,PMMoV was first identified in Fengcheng City by using DAS-ELISA and RT-PCR method,and named as PMMoV Fengcheng isolate (PMMoV-FC).Three pairs of specific primers were used to amplify the complete genome sequence of PMMoV-FC,and the results of sequence analysis and phylogenetic analysis showed that PMMoV-FC was closely related with 9 PMMoV isolates reported in China or abroad with a sequence identity of between 94.3％-99.7％.Phylogenetic tree was constructed,showing that PMMoV-FC was closely related with Chinese domestic isolate,Japanese isolate and southern American isolate but not with South Korean isolate or Spanish isolate.Due to the significant losses caused by PMMoV to pepper,further research is required to control and prevent the prevailing of the diseases caused by PMMoV-FC in Liaoning Province.