蒙氏假单胞菌3A菌株对烟草漂浮育苗中TMV的钝化效果

摘要

Pseudomonas monteilii 3A strain was selected to evaluate the passivation effect on Tobacco mosaic virus (TMV) in tobacco seedlings by real-time RT-PCR,combined with the incidence and disease index of the virus disease.The results showed that P.monteilii 3A bacterial liquid with different concentration gradients (200,400 and 600 ×) had significant passivation effects on TMV in the residual dry roots and leaves of the old seedling sheds and trays.Meanwhile,using the clipping tool treated with P.monteilii 3A bacterial liquid could reduce the incidence and disease index of the virus disease,and the relative control effect was 51.42％-100％.P.monteilii 3A bacterial liquid could reduce the amount of TMV in the matrix polluted with TMV through bio-passivation by 72.73％-88.46％,and the relative control effect on the virus disease was 61.56％-87.46％.The best dilution of P.monteilii 3A bacterial liquid was 1:200,and the optimum contact time on old seedling sheds and trays was no less than 20 min.Meanwhile,the clipping tools should be treated more than 1 min.%选用对烟草花叶病毒Tobacco mosaic virus (TMV)具有显著拮抗活性的蒙氏假单胞菌Pseudomonas manteilii 3A菌株对烟草漂浮育苗过程中被TMV污染的育苗棚、育苗盘、基质及剪叶机械等进行生物钝化,并利用real-time RT-PCR对污染基质中TMV的含量进行了定量检测.结果显示,200、400倍及600倍P.monteilii 3A菌悬液稀释液均可显著钝化病苗残留干叶及干根中的TMV;剪叶机械经不同稀释倍数的P.monteilii 3A菌悬液处理后,在一定程度上抑制了TMV的发生,相对防效达51.42％～100％;基质经不同稀释倍数的P.monteilii 3A菌悬液处理后,其中TMV的含量也下降了72.73％～88.46％,对TMV的相对防效达61.56％～87.46％.其中,200倍P.monteilii 3A稀释液处理育苗棚、育苗盘20 min以上,浸泡剪叶机械1min以上对TMV钝化效果最好.