超高效液相色谱-串联质谱法测定豇豆中8种农药残留

摘要

本文建立了超高效液相色谱-串联质谱法(UPLC-MS/MS)同时测定豇豆中啶虫脒、阿维菌素、甲氨基阿维菌素苯甲酸盐、氟啶虫胺腈、灭蝇胺、苯醚甲环唑、吡唑醚菌酯和烯酰吗啉残留量的方法.样品经乙腈提取,采用ACQUITY_UPLCTMBEH C18色谱柱分离,应用UPLC-MS/MS正离子扫描进行定性定量分析.结果表明,在0.01～1 μg/mL范围内,8种农药色谱峰面积与浓度均呈线性相关;3个添加水平下,回收率均在90％以上;8种农药在豇豆中的检出限为0.08～1.81 μg/kg,定量限为0.024～0.603 μg/kg.在实际豇豆样品检测中检测到5种农药残留,本方法灵敏度高、速度快,可为检测豇豆中8种农药残留量提供参考.%An analytical method based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to analyze residues of acetamiprid,abamectin,emamectin benzoate,sulfoxaflor,cyromazine,difenoconazole,pyraclostrobin and dimethomorph in cowpea.The samples were homogenized and extracted with acetonitrile,the retention of target compounds was performed on an ACQUITY_UPLCTMBEH C18 column by gradient elution,the qualification and quantification were analyzed by spectrometry with electrospray ionization in positive mode.The results showed that the chromatographic peak area was linearly related to the concentration of pesticides in the range of 0.01-1 μg/mL.Under the 3 adding levels,the recovery rate is above 90％.The limits of detection (LOD) and quantitation (LOQ) were 0.08-1.81 μtg/kg and 0.024-0.603 μg/kg,respectively.5 pesticides residues were detected in the actual cowpea samples.Taken together,the present study suggest that UPLC-MS/MS method with high sensitivity and high speed can be recommended to be used for the analysis of the residues of 8 pesticides in cowpea.