温度胁迫下烟粉虱MED隐种与亚洲Ⅱ3隐种三个抗寒基因表达模式的分析

摘要

烟粉虱Bemisia tabaci具有强的温度耐受性,但烟粉虱入侵种MED隐种与本地种亚洲Ⅱ 3隐种在我国的地理分布上存在巨大的差异.因此本研究以烟粉虱MED隐种和亚洲Ⅱ3隐种为研究对象,采用qRT-PCR技术分别检测两种烟粉虱3个抗寒基因(erythrocyte binding protein,trehalase,dopadecarboxylase)在5个梯度温度胁迫下mRNA水平上的相对表达量,分析其在高低温胁迫过程中表达量的变化,探索抗寒基因在烟粉虱MED隐种成功入侵并广泛分布于各气候区域中所起的作用.研究结果表明这两种烟粉虱隐种体内,3个抗寒基因均显著受低温诱导.但相对于Asia Ⅱ 3隐种,低温胁迫下烟粉虱MED隐种体内的3种抗寒基因能更快速地做出响应,且基因上调或下调的量也相对较多.%Bemisia tabaci has strong temperature tolerance (heat resistance and cold resistance).However,there were significant differences in the distribution of invasive species (MED cryptic species) and native species (Asia Ⅱ 3 cryptic species) in China.Therefore,in this study,we used qRT-PCR to detect the mRNA relative expression level of 3 important cold-resistant genes encoding the erythrocyte binding protein,trehalase and dopadecarboxylase in the two B.tabaci cryptic species under the stress of five gradient temperatures.The purposc of the study is to explore the roles of cold-resistant genes in the successful invasion of B.tabaci MED cryptic species and widespread distribution in different climatic regions by analyzing the change of the relative expression level.The results of qPCR test showed that three cold-resistant genes were significantly induced by low temperature in the two B.tabaci cryptic species.Compared with the Asia Ⅱ 3 cryptic species,B.tabaci MED could make adjustments to adapt to the low-temperature environment more quickly,and the adjustment level was also relatively higher.Therefore,we speculate that it may be one of the important mechanisms that makes MED cryptic species invade into a wide range of cold regions and adapt quickly to survive.