利用BSMV Agro/LIC进行小麦条锈菌耐高温相关基因功能分析的互作体系构建

摘要

In order to characterize the biological functions of the high-temperature tolerance-related genes of Puccinia striiformis f.sp.tritici (Pst) by using HIGS method,it is necessary to develop a compatible interactive system containing wheat variety and Pst pathogen and Barley stripe mosaic virus (BSMV).In this study,20 varieties (highly susceptible when inoculated at 10℃) were inoculated with 22 high-temperature-tolerant isolates at 21℃ and their compatibility levels were evaluated,and we found 11 highly susceptible varieties among the tested ones and 3 isolates with a highly stable level of high-temperature tolerance and a wider virulence spectrum.Based on the results,6 varieties such as ‘Local Red’ were compatible with the BSMV strain containing a TaPDS fragment.Therefore,the combinations of the variety ‘Local Red’ with the isolates like 15335-2 and 15323-4 were the best interactive systems for characterizing high temperature-tolerance-related genes of Pst with BSMV Agro/LIC.%为利用HIGS技术研究条锈菌耐高温胁迫相关基因的功能,需要构建小麦品种与条锈菌及大麦条纹花叶病毒Barley stripe mosaic virus (BSMV)亲和互作体系.本研究选用20个常温下(10℃)高感条锈病的小麦品种和22个耐高温条锈菌菌株,进行高温(21℃)条件下接种亲和性分析,发现11个品种高感条锈病、3个菌株耐高温性稳定且毒性谱较宽.在此基础上,利用含有小麦TaPDS基因片段的重组BSMV侵染上述候选小麦品种,确定‘Local Red’等6个品种与BSMV亲和程度符合试验要求.筛选出小麦品种‘Local Red’与菌株15335-2、15323-4等组成的最佳互作组合,适合利用BSMVAgro/LIC方法开展小麦条锈菌耐高温相关基因功能的研究.