正交试验优化酶法提取黄芪总黄酮工艺

摘要

Abstrcts:Cellulase enzymolysis assisted ethanol extraction of astragalus total flavonoids from Astragalus membranaceus was optimized in the paper .To observe and study this ,the effects of factors including enzymatic content ,enzymolysis time ,solid-to-liquid ratio ,ethanol concentration ,extraction time ,extraction temperature on the extraction rates of astragalus total flavonoids were explored by single-factor and orthogonal test .The results showed that solid-to-liquid ratio was the most critical factor affecting the extraction rate of astragalus total fla-vonoids,followed by ethanol concentration and extraction temperature ,extraction time exhibited less effect on extraction rate of astragalus total flavonoids .The optimal extraction conditions were solid-to-liquid ratio of 30mL/g,ethanol concentration of 90%,extraction temperature of 75℃,extraction time of 2.5h after cellulase enzymolysis pretreatment in the conditions of enzymatic content of 7.5μL/g,enzymolysis pH4.0,enzymolysis temperature 55℃,enzymolysis time of 1.5h,astragalus total flavonoids extraction was up to 0.57%and impro-ving 46 .15%compared with the traditional ethanol extraction of 0 .39%.%　　以蒙古黄芪为试验材料，研究纤维素酶解辅助乙醇提取黄芪总黄酮的工艺条件。考察了酶添加量、酶解时间、液固比、乙醇浓度、提取时间、提取温度6个单因素对黄芪总黄酮提取率的影响，在单因素试验的基础上，选择四因素四水平进行正交试验优化工艺参数。结果表明：蒙古黄芪在纤维素酶添加量7．5μL/g，pH值4．0，温度55℃，酶解预处理1．5h后，液固比对黄芪总黄酮提取率影响显著，其次是提取时间和乙醇浓度，提取温度对黄芪总黄酮提取率的影响相对较小。在液固比30 mL/g ，乙醇浓度90％，提取温度75℃，提取时间2．5 h的最优工艺条件下，黄芪总黄酮的提取率为0．57％。与传统醇提法（0．39％）相比，黄芪总黄酮提取率提高了46．15％。