新型海洋硫酸多糖DPS对大鼠脑缺血的保护作用

摘要

采用开颅机械闭塞法阻断大鼠大脑中动脉(Middle cerebral artery occlusion，MCAO)造成大鼠局灶性脑缺血模型，观察了DPS(D-polysaccharide sulfate)对MCAO大鼠局灶性脑缺血的保护作用。结果发现，iv DPS剂量分别为20、40me/kg于术后30min静脉注射，能明显缩小MCAO24h造成的脑梗塞范围，减少脑水含量，但对神经功能障碍无明显的改善效果，同时DPS具有抑制血小板聚集、减少血栓素(TXA2)生成和提高ATⅢ(AntithrombinⅢ)活性的作用。提示DPS可通过抗凝抗血小板聚集、减少TXA2释放来发挥其抗脑缺血的作用。%Effects of DPS,a marine heparinoids, against cerebral ischemia induced by occlusion of middle cerebral artery are studied. The results showed that DPS at concentration of 20 and 40mg/kg significantly decreased cerebral infarcted area, reduced water contents of brain tissue. Moreover, DPS significantly enhanced ATⅢ activity and exerted inhibitory effects on platelet aggregation characterized by the blockade of release TXA2. These findings indicated that DPS could protecte ischemic brain tissue induced by MCAO, chiefly through promoting AT Ⅲ activity, inhibiting platelet aggregation,and down-regulation of TXA2 formation.