孤雌生殖海带对草丁膦的敏感性

摘要

In the experiment, young sporophytes of parthenogenetic L ami naria japonica were divided into three groups according to their total lengths : a. (5.00±0.06)mm, b. (9.50±0.10)mm and c. (16.10±0.13)mm, and were treated with 13 concentrations of basta solution in each group respectively. Basta dose are 0-21.2μg/ml. LD50 at 36， 48, 60 and 72 hours were calculated. R esults showed that LD50 of sporophytes of parthenogenetic L. japonic a (0.5-1.6)cm to basta is not correlated with its length. The sporophytes of parthenogenetic L. japonica are more sensitive to basta than to chlorampheni col and hygromycin that have been used as selection pressure in genetic engineer ing of L. japonica. Our observation suggests that bar gene which encodes pho sphinothricin-N-acetyltrensferase can be a more efficient selectable marker ge ne.%于1999年4-7月，以孤雌生殖海带为材料，运用统计学方法研究其对 草丁膦的敏感性，以期得到一定剂量和时间范围内草丁膦对不同长度孤雌生殖海带的半数致 死剂 量(LD50)。结果表明，孤雌海带在0.5-1.6cm长度范围内，草丁膦的半数致死剂 量与海带的长度不相关，而与草丁膦的剂量和作用时间相关。孤雌生殖海带对草丁膦比对氯 霉素 和潮霉素更为敏感，提示bar基因有可能成为海带基因工程更为理想的选择标记基因。