以大型经济褐藻裙带菜配子体为实验材料,采用活性检测法对雌诱激素在有性生殖过程中的作用进行了研究。结果表明,刚刚排出的雌配子分泌了一种或几种易挥发的激素类物质,这些激素类物质能够促进精子释放并吸引精子向其游动,完成受精作用。另外,通过显微计数释放精子数的方法对影响裙带菜雌诱激素分泌的培养时间和培养的光照强度、环境温度、光周期等进行了实验,对精卵结合过程中雌诱激素分泌的最佳条件进行了研究,发现雌配子体在发育培养6—8d时检测到雌诱激素活性,最佳的温度条件为19℃,在光强40—160μmol/（m2·s）范围均%The roles of male-gametophytes in Undaria pinnatifida during its sexual reproduction were studied. The results showed that eggs were able to secrete different kinds of volatile organic substances. And these substances were found able to stimulate the spermatophore to release sperms, as well as to lead sperms swimming toward the eggs to accomplish fertilization. In this study, several environmental factors causing secretion of sperm attractants were also discovered. These factors included： cultivation time, temperature, light irradiance and photoperiod. The results showed that the activation of sperm attractants are at the highest when the female gametophytes developed for 6 to 8 days. The optimal environmental conditions for sperm attractants secretion were found at temperature 19℃, photoperiod L ： D = 12h ： 12h, light irradiance 40—160μmol/（m2·s）.
展开▼
