2株南极海洋寡营养细菌（Alteromonas stellipolaris）脂多糖对三疣梭子蟹（Portunus trituberculatus）非特异性免疫活性的影响

摘要

The indices of non-specific immunity were measured in 3, 6, 9, 12 days after Portunus trituberculatus was treated with lipopolysaccharides （LPS） extracted from two Antarctic ultramicro-bacteria Alteromonas stellipolaris, AT52 and AT82. The results showed those LPS were able to improve the activities of anti-bacteria, lysozme （LSZ）, phenoloxidase （PO）, superoxide dismutase （SOD）, and peroxidase （POD） significantly （P0.05）. A variety of enzyme activities reached maximum levels at different days, and AT82 LPS injection group was found better than those of AT52 LPS injection group. Our studies suggested LPS of Antarctic ultramicro-bacteria is a desirable potential immunologic stimulant for P. trituberculatus to fight for bacterial diseases. Future studies are needed to understand the mechanism of the immunity response and the best dosage for these LPS.%从2株南极海洋寡营养细菌（Alteromonas stellipolaris,菌株AT82和AT52）中提取脂多糖（Lipopolysaccharide,LPS）,分不同处理组免疫注射三疣梭子蟹,在免疫后第3、6、9、12天分别检测梭子蟹血清的抗菌活力、溶菌酶活力、超氧化物歧化酶活力、酚氧化酶活力和过氧化物酶活力等非特异性免疫指标的变化。结果表明,自2株南极寡营养细菌提取的脂多糖均能显著提高梭子蟹血清中的抗菌活力、溶菌酶活力、超氧化物歧化酶活力、酚氧化酶活力和过氧化物酶活力等指标（P〈0.05）。各种酶活