采用60Co-γ射线诱变和高温胁迫处理条斑紫菜野生品系（geT），进行了耐高温品系的选育研究。结果表明，在18℃和22℃组，所选育的耐高温品系（TM-18）和WT品系的壳孢子存活率无显著差异，但在24℃和25℃组，TM-18与胛相比，壳孢子存活率分别提高了274％-296％，畸形苗率分别下降了286％和161％，假根发生率分别提高了151％和429％。将18℃培养50d的苗再分别在18℃、22℃和24℃下再培养30d，TM-18苗的绝对生长率分别为WT苗的3．82、3．16和4．95倍，特定生长率为WT苗的1．53、1．49、2．10倍。在24℃下培养15d或25℃下培养10d，WT苗停止生长，出现了卷曲和严重腐烂，而TM-18苗仍然生长良好，不腐烂。另外，TM-18的三种主要光合色素（Chl．a、PE和Pc）含量明显比WT高。上述结果说明，TM-18是一个颜色好、生长快和耐高温的优良品系。%A high-temperature-tolerant strain （TM-18） ofPorphyra yezoensis Ueda was isolated from the wild type （WT） using by 60Co-T-radiation and high-temperature cultivation. At 18 and 22℃, there was no significant difference on the sur- vival rates between the WT and TM-18 conchospores. However, at 24 and 25 ℃C, the survival rates and the rhizoid germination rates of conchospore-germlings of TM-18 were 274% and 296%, 151% and 429% higher than those of the WT, respectively. Furthermore, the occurrence rates of abnormal blades in TM-18 was reduced by 286% and 161% compared with the WT. When the 50-day-old conchospore-germlings initially cultured at 18℃ were cultured at 18, 22 and 24℃ for another 30 days, the specific growth rates of the TM-18 blades were 3.82, 3.16 and 4.95 times higher than the WT, respectively, and the absolute growth rates of the TM-18 blades were 1.53, 1.49 and 2.10 higher than the WT, respectively. The WT blades stopped growing and then severely decayed after being cultured for 15 days at 240C or 10 days at 25℃. In comparison, the TM-18 blades maintained normal growth. Finally, the contents of the three main photosynthetic pigments （Chl.a, PE and PC） in the TM-18 blades were significantly higher than those of the WT blades. This study showed that the TM-18 strain was superior to WT strain in growth, high-temperature tolerance, and pigment productions.
展开▼
