Cd2＋与Zn2＋的联合效应对马氏珠母贝（Pinctada martensii）受精率的影响

摘要

Using the central composite face-centered design （CCF） and response surface methodology, the combined effects of Cdz＋ （0.030--20.000mg/L） and Zn2＋ （0.100--20.000mg/L） on the fertilization rate in Pinctada martensii were studied under laboratory conditions. The results showed that the effects of two factors Cd2＋ and Zn2＋ on the fertilization rate in P. martensii were highly significant （P〈0.01）, and effect of Cd2＋ was more significant than that of Zn2＋. The interaction between the two factors was also highly significant （P〈0.01）. Based on the response surface plots, it was found that with the increased concentration of Cd2＋ and Zn2＋ , the fertilization rate was decreased. When Zn2＋ concentration was at its lowest level （0.100mg/L）, the fertilization rate decreased with the increased Cd2＋ concentration. When Cd2＋ concentration was at its lowest level （0.030mg/L）, the fertilization rate was first decreased and then increased as the concentration of Zn2＋ increased. The model equation of the fertilization rate towards Cd2＋ and Zn2＋ was established, with R2 = 0.996, Adj.RE = 0.987 and Pred.R2 = 0.887 （P〈0.01）. The model equation could be practically used for forecasting the effects of Cd2＋ and Zn2＋ on the fertilization rate in P. martensii.%采用中心组合设计和响应曲面法在实验室条件下研究了Cd2＋与Zn2＋对马氏珠母贝受精率的影响。cd2＋、Zn2＋的浓度范围分别为0．030-20mg／L、0．100-20mg／L。结果表明：Cd2＋与Zn2＋两因子对马氏珠母贝受精率的一次效应极显著（P〈0．01）；Cd2＋与Zn2＋两因子一次互作效应极显著伊〈0．01）。经响应曲面＋浓度的上升而下降；当Cd2＋浓度在最低浓度（0．030mg／L）时，受精率随Zn2＋浓度的上升而先下降后升高。本实验建立了马氏珠母贝受精率与Cd2＋、Zn2＋间关系的模型方程（R^2=0．996，Adj．R^2=0．987，Pred．R^2=0．887，P〈0．01）并可用于预测Cd2＋、Zn2＋对马氏珠母贝受精率的影响。