The potential economic fish Acrossocheilus fasciatus has obvious advances for studies on the development of embryo, larva and juvenile. As far as we know, the development of this species remains unknown. In our current work, we aim to reveal how the embryonic, larval and juvenile development via artificial insemination in A. fasciatus. The results show that the fertilized egg of A. fasciatus is in shape of spherical, featured by sinking and slight-adhesive. When the temperature is 23--25℃, the duration of hatching lasts 46h 45min. The process of embryonic development undergoes the following 7 stages： blastoderm, cleavage, blastula, gastrula, neurula, organogenesis and hatching. When the temperature is 24--27℃, the duration of larval and juvenile development lasts 22 days. The newly hatched larvae have big yolk sac and the rudiment of pectoral fin and anus are formed at this stage. With the decreasing of yolk sac, the larvae begin to ingestion and transform to mixed feeding stage at 4 DAH （day after hatching）. 3 days later, the yolk sac of larvae exhaust absolutely and enter into the exogenous feeding stage, meanwhile eight pigment strips and fins are all formed. Since then the larval development steps to the late larvae stage. As the larvae grow to the 14 DAH, most fin rays are formed integrity. The for- mation of scales starts from the 16 DAH and entirely complete at the 22 DAH. From now on, the ontogeny ofA. fasciatus gets to the fingerling stage.%采用干法授精方法获得受精卵，在人工培育条件下观察了光唇鱼胚胎及仔、稚鱼发育过程。结果表明，光唇鱼受精卵呈圆球形，沉性、弱粘性。在水温23—25℃下，胚胎发育历时46h45min，经历了胚盘形成、卵裂、囊胚、原肠胚、神经胚、器官形成和孵化等阶段。在水温24～27℃下，仔、稚鱼发育历时22d，初孵仔鱼具有较大的卵黄囊，胸鳍原基及肛门原基形成；4日龄仔鱼开口摄食，进入混合营养期；7日龄仔鱼卵黄囊消失，营外源性营养，体侧8条横斑形成，各鳍均已出现，进入晚期仔鱼阶段；14日龄仔鱼各鳍鳍条发育基本完成；16日龄仔鱼鳞片开始出现，进入稚鱼期：22日龄稚鱼全身被鳞，进入幼鱼期。
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