条石鲷（Oplegnathus fasciatus）心脏早期发育的组织学观察

摘要

Based on the histological observation under optical microscope, we studied the ontogeny of the heart of rock bream Oplegnathusfasciatus at （24 _＋ 1.0）~C. At 13h 30min postfertilization, the heart primordium, an ellipsoidal tube de- rived from the mesoderm, appears in the ventral side of the midbrain. It consists of I-YSL exteriorly and a stuff of spindle cells interiorly. At 20h 30min post-fertilization, the heart is beginning to beat and the blood starts to circulate through a closed set of channels. At hatching, the heart appeared as a straight tube lying anterior to the coelom of rock bream. At 2 DAH （days after hatching）, the heart was differentiated into four parts： sinous venosus, atrium, ventricle and bulbous arte-riosus. At 3 DAH, the atrioventricular and semilunar valves were formed. At 6 DAH, the sinoatial valve was developed and the trabeculae in the ventricle appeared. At 12 DAH, the blood cells in the heart increased sharply. At 27 DAH, the trabeculae were appeared in the atrium, and the heart of rock bream was developed completely.%应用石蜡切片技术和显微测量法，对条石鲷心脏的早期发生、分化和形成过程进行了连续观察。结果表明，在水温（24±1．0）℃的条件下，心脏原基在受精后13h30min出现于中部脑的腹面，其外部由卵黄多核层包围，内部则为扁平细胞所填充；受精后20h30min，心脏开始搏动，可见血细胞：初孵仔鱼心脏呈直管状，位于体腔前端；2日龄（days after hatching，DAH），心脏分化成静脉窦、心耳（心房）、心室和动脉球四个部分；3日龄，耳室瓣和半月瓣形成；6日龄，窦耳瓣和心小室形成：12日龄，心脏内血细胞的数量急剧增加，血流量显著加大；27日龄，心耳内形成小梁结构，心脏发育完善。